Scholz R, Meinhof W
Department of Dermatology, Medical Faculty, Technical University Aachen, Germany.
Mycoses. 1991 Sep-Oct;34(9-10):411-4. doi: 10.1111/j.1439-0507.1991.tb00804.x.
Two hundred and seventy isolates of Trichophyton rubrum from 182 patients were subjected to susceptibility testing with griseofulvin according to Granade & Artis. A slight modification of the method concerning homogenization procedures gave better growth of controls in our investigation. In 245 cases an MIC could be determined. Among these isolates, seven strains from six patients presented MICs between 2 and 3 micrograms griseofulvin per ml, a value which, according to Artis et al., is to be considered as relatively resistant. The other isolates proved to be more susceptible to griseofulvin. Three of the patients showed no indication of therapeutic failure of griseofulvin treatment. The other three patients had onychomycoses of long standing and had been treated with griseofulvin previously with little or limited success. It is our conclusion that griseofulvin susceptibility testing is not a reliable instrument in explaining failure of griseofulvin therapy in Trichophyton rubrum mycoses.
根据格拉纳德和阿蒂斯的方法，对来自182名患者的270株红色毛癣菌分离株进行了灰黄霉素药敏试验。在我们的研究中，对匀浆程序的方法进行了轻微修改，使对照菌生长得更好。在245例病例中可以确定最低抑菌浓度（MIC）。在这些分离株中，来自6名患者的7株菌株的MIC为每毫升2至3微克灰黄霉素，根据阿蒂斯等人的说法，该值应被视为相对耐药。其他分离株对灰黄霉素更敏感。其中3名患者未显示灰黄霉素治疗失败的迹象。其他3名患者患有长期甲癣，之前接受过灰黄霉素治疗，但效果甚微或有限。我们的结论是，灰黄霉素药敏试验在解释红色毛癣菌病中灰黄霉素治疗失败方面不是一个可靠的手段。
