Granade T C, Artis W M
J Clin Microbiol. 1982 Dec;16(6):1043-7. doi: 10.1128/jcm.16.6.1043-1047.1982.
A microculture broth assay system for griseofulvin susceptibility testing of Trichophyton rubrum was further characterized. The effects of mass and number of colony-forming units of a fragmented mycelial inoculum, 5- or 8-day incubation periods, 25 or 32 degrees C incubation temperatures, and the solvents used to dissolve griseofulvin on the minimal inhibitory concentration (MIC) of griseofulvin were determined. An inoculum density with an absorbance of 0.600 at 450 nm ensured successful inoculation of all microcultures. Reduction of the inoculum mass to an absorbance of 0.200 lowered the number of colony-forming units in the inoculum by 60 to 80%. This decreased the efficiency of inoculation but did not alter the resulting MIC. There was no correlation between MIC and the number of colony-forming units used to initiate growth. Neither incubation temperature nor the length of incubation affected the MIC. The use of either acetone or ethanol to solubilize griseofulvin likewise had no effect on the MIC. The mean reproducibility of the MICs determined with the microculture method was 96%.
对用于红色毛癣菌灰黄霉素敏感性测试的微量培养肉汤测定系统进行了进一步表征。确定了破碎菌丝体接种物的菌落形成单位的质量和数量、5天或8天的培养期、25或32摄氏度的培养温度以及用于溶解灰黄霉素的溶剂对灰黄霉素最低抑菌浓度（MIC）的影响。在450nm处吸光度为0.600的接种密度可确保所有微量培养物成功接种。将接种物质量降低至吸光度为0.200可使接种物中的菌落形成单位数量减少60%至80%。这降低了接种效率，但未改变所得的MIC。MIC与用于起始生长的菌落形成单位数量之间没有相关性。培养温度和培养时间均不影响MIC。使用丙酮或乙醇溶解灰黄霉素同样对MIC没有影响。用微量培养法测定的MIC的平均重现性为96%。
