Dynamic combinatorial mass spectrometry leads to metallo-beta-lactamase inhibitors.
作者信息
Liénard Benoît M R, Hüting Rebekka, Lassaux Patricia, Galleni Moreno, Frère Jean-Marie, Schofield Christopher J
机构信息
Chemistry Research Laboratory and OCISB, Oxford, UK.
出版信息
J Med Chem. 2008 Feb 14;51(3):684-8. doi: 10.1021/jm070866g. Epub 2008 Jan 19.
PMID:18205296
Abstract
The use of protein ESI mass spectrometry under non-denaturing conditions to analyze a dynamic combinatorial library of thiols/disulfides with the BcII metallo-beta-lactamase enabled the rapid identification of an inhibitor with a K(i) of < 1 microM. The study exemplifies the utility of protein-MS for screening dynamic mixtures of potential enzyme-inhibitors.
摘要
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