Liénard Benoît M R, Hüting Rebekka, Lassaux Patricia, Galleni Moreno, Frère Jean-Marie, Schofield Christopher J
Chemistry Research Laboratory and OCISB, Oxford, UK.
J Med Chem. 2008 Feb 14;51(3):684-8. doi: 10.1021/jm070866g. Epub 2008 Jan 19.
The use of protein ESI mass spectrometry under non-denaturing conditions to analyze a dynamic combinatorial library of thiols/disulfides with the BcII metallo-beta-lactamase enabled the rapid identification of an inhibitor with a K(i) of < 1 microM. The study exemplifies the utility of protein-MS for screening dynamic mixtures of potential enzyme-inhibitors.
