Okahashi N, Koga T, Akada H, Hamada S
Infect Immun. 1983 Feb;39(2):552-8. doi: 10.1128/iai.39.2.552-558.1983.
The serotype-specific antigen of Streptococcus sanguis ST3 (serotype I, biotype A) was extracted, chromatographically purified, and characterized by immunological and chemical methods. The antigen was extracted from purified cell walls with hot trichloroacetic acid, followed by ion-exchange chromatography on a DEAE-Sephadex A-25 column and gel filtration through a Sephadex G-100 column. A peak fraction was obtained that gave a single precipitin band when reacted with anti-type I serum. The type I antigen was a polysaccharide composed of glucose, rhamnose, and N-acetylglucosamine in a molar ratio of 1.4:2.5:1.0. Quantitative precipitin inhibition tests with various haptenic sugars indicated that an alpha-glucosidic linkage is the immunodeterminant of the type I antigen.
血链球菌ST3（血清型I，生物型A）的血清型特异性抗原被提取、经色谱法纯化，并通过免疫学和化学方法进行表征。该抗原用热三氯乙酸从纯化的细胞壁中提取，随后在DEAE-葡聚糖A-25柱上进行离子交换色谱，并通过葡聚糖G-100柱进行凝胶过滤。得到一个峰级分，当与抗I型血清反应时产生单一沉淀带。I型抗原是一种多糖，由葡萄糖、鼠李糖和N-乙酰葡糖胺以1.4:2.5:1.0的摩尔比组成。用各种半抗原糖进行的定量沉淀抑制试验表明，α-糖苷键是I型抗原的免疫决定簇。
