Kanebo Cosmetics Inc., Basic Research Laboratory, Kanagawa, Japan.
Skin Res Technol. 2010 May;16(2):137-41. doi: 10.1111/j.1600-0846.2009.00410.x.
BACKGROUND/PURPOSE: Dermal water plays an important role in the physical properties of the skin. Recently, researchers have attempted to directly measure the dermal water content in vivo using magnetic resonance imaging, near infrared spectroscopy, and Raman spectroscopy. However, these methods have limitations. Although confocal Raman spectroscopy has been developed to measure the water content in the skin, no reports have suggested that this instrument can measure the dermal water content. This report describes a method for measuring the dermal water content in vivo using confocal Raman spectroscopy.
We used a confocal Raman spectrometer and adjusted the laser exposure time and depth increments according to the skin depth. Age-related changes in the dermal water content of the forearm were examined in 30 young and 30 elderly male subjects. Diurnal changes in the dermal water content of the forearm were examined in 12 elderly male subjects.
Adjusting the exposure time and depth increment dramatically improved the signal-to-noise ratios of the Raman spectra. Elderly dermis had significantly higher water content than young dermis. Moreover, the dermal water content displayed a diurnal change.
This study demonstrates that the dermal water content can be measured in vivo using confocal Raman spectroscopy.
背景/目的：皮肤水分在皮肤的物理性质中起着重要作用。最近，研究人员试图使用磁共振成像、近红外光谱和拉曼光谱直接测量体内皮肤水分含量。然而，这些方法存在局限性。尽管已经开发出共焦拉曼光谱来测量皮肤中的水分含量，但没有报道表明该仪器可以测量皮肤水分含量。本报告描述了一种使用共焦拉曼光谱术测量体内皮肤水分含量的方法。
我们使用共焦拉曼光谱仪，并根据皮肤深度调整激光暴露时间和深度增量。检查了 30 名年轻男性和 30 名老年男性前臂皮肤水分的年龄相关性变化。检查了 12 名老年男性前臂皮肤水分的昼夜变化。
调整暴露时间和深度增量可显著提高拉曼光谱的信噪比。老年真皮的水分含量明显高于年轻真皮。此外，皮肤水分含量显示出昼夜变化。
本研究表明，共焦拉曼光谱术可用于测量体内皮肤水分含量。
