Song Yiqiang, Yan Mingquan, Muneoka Ken, Chen YiPing
Department of Cell and Molecular Biology, Tulane University, New Orleans, Louisiana, USA.
Dev Dyn. 2008 Feb;237(2):411-6. doi: 10.1002/dvdy.21427.
The anterior eye chamber and the kidney capsule of the mouse have been traditionally used for long-term culture of tooth germ grafts. However, although these sites provide an excellent growth environment, they do not represent real in situ sites for the development of a grafted tooth germ. Here, we describe a protocol to transplant a tooth germ into the mandibular diastema region of mouse embryos using exo utero surgery. Our results demonstrate that the mouse embryonic diastema region represents a normal physiological environment for the development of transplanted tooth germs. Transplanted tooth germs developed synchronically with and became indistinguishable from the endogenous ones. These ectopic teeth were vascularized and surrounded with nerve fibers, and were able to erupt normally. Thus, the exo utero transplantation approach will provide a new avenue to study tooth development and regeneration.
传统上，小鼠的眼前房和肾包膜一直用于牙胚移植物的长期培养。然而，尽管这些部位提供了良好的生长环境，但它们并非移植牙胚发育的真正原位部位。在此，我们描述了一种使用宫外手术将牙胚移植到小鼠胚胎下颌间隙区域的方案。我们的结果表明，小鼠胚胎间隙区域代表了移植牙胚发育的正常生理环境。移植的牙胚与内源性牙胚同步发育，且难以区分。这些异位牙有血管供应并被神经纤维包围，并且能够正常萌出。因此，宫外移植方法将为研究牙齿发育和再生提供一条新途径。
