Koegl Manfred, Uetz Peter
Translational Research, and Genomics and Proteomics Core Facilities, German Cancer Research Center, Im Neuenheimer Feld 515, 69120 Heidelberg, Germany.
Brief Funct Genomic Proteomic. 2007 Dec;6(4):302-12. doi: 10.1093/bfgp/elm035. Epub 2008 Jan 24.
Yeast two-hybrid (Y2H) screening methods are an effective means for the detection of protein-protein interactions. Optimisation and automation has increased the throughput of the method to an extent that allows the systematic mapping of protein-protein interactions on a proteome-wide scale. Since two-hybrid screens fail to detect a great number of interactions, parallel high-throughput approaches are needed for proteome-wide interaction screens. In this review, we discuss and compare different approaches for adaptation of Y2H screening to high-throughput, the limits of the method and possible alternative approaches to complement the mapping of organism-wide protein-protein interactions.
酵母双杂交（Y2H）筛选方法是检测蛋白质-蛋白质相互作用的有效手段。优化和自动化已将该方法的通量提高到一定程度，使得在全蛋白质组范围内系统地绘制蛋白质-蛋白质相互作用成为可能。由于双杂交筛选无法检测到大量的相互作用，因此全蛋白质组相互作用筛选需要并行的高通量方法。在本综述中，我们讨论并比较了将Y2H筛选适用于高通量的不同方法、该方法的局限性以及用于补充全生物体蛋白质-蛋白质相互作用图谱绘制的可能替代方法。
