Watanabe Hiroshi, Mogushi Kaoru, Miura Masahiko, Yoshimura Ryo-Ichi, Kurabayashi Tohru, Shibuya Hitoshi, Tanaka Hiroshi, Noda Shuhei, Iwakawa Mayumi, Imai Takashi
Department of Oral Restitution, Tokyo Medical and Dental University, Tokyo, Japan.
Radiother Oncol. 2008 May;87(2):237-42. doi: 10.1016/j.radonc.2007.12.027. Epub 2008 Jan 25.
The management of lymphatic metastasis of early-stage oral tongue carcinoma patients is crucial for its prognosis. The purpose of this study was to evaluate the predictive ability of lymphatic metastasis after brachytherapy (BRT) for early-stage tongue carcinoma based on gene expression profiling.
Pre-therapeutic biopsies from 39 patients with T1 or T2 tongue cancer were analyzed for gene expression signatures using Codelink Uniset Human 20K Bioarray. All patients were treated with low dose-rate BRT for their primary lesions and underwent strict follow-up under a wait-and-see policy for cervical lymphatic metastasis. Candidate genes were selected for predicting lymph-node status in the reference group by the permutation test. Predictive accuracy was further evaluated by the prediction strength (PS) scoring system using an independent validation group.
We selected a set of 19 genes whose expression differed significantly between classes with or without lymphatic metastasis in the reference group. The lymph-node status in the validation group was predicted by the PS scoring system with an accuracy of 76%.
Gene expression profiling using 19 genes in primary tumor tissues may allow prediction of lymphatic metastasis after BRT for early-stage oral tongue carcinoma.
早期口腔舌癌患者淋巴转移的管理对其预后至关重要。本研究旨在基于基因表达谱评估近距离放射治疗（BRT）后早期舌癌淋巴转移的预测能力。
使用Codelink Uniset Human 20K生物芯片对39例T1或T2期舌癌患者的治疗前活检组织进行基因表达特征分析。所有患者均接受低剂量率BRT治疗其原发性病变，并在观察等待策略下接受严格随访以观察颈部淋巴转移情况。通过置换检验在参考组中选择用于预测淋巴结状态的候选基因。使用独立验证组通过预测强度（PS）评分系统进一步评估预测准确性。
我们选择了一组19个基因，其在参考组中有或无淋巴转移的类别之间表达差异显著。PS评分系统预测验证组的淋巴结状态，准确率为76%。
使用原发性肿瘤组织中的19个基因进行基因表达谱分析可能有助于预测早期口腔舌癌BRT后的淋巴转移。
