Fairfax Kirsten A, Kallies Axel, Nutt Stephen L, Tarlinton David M
The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville 3050, Australia.
Semin Immunol. 2008 Feb;20(1):49-58. doi: 10.1016/j.smim.2007.12.002. Epub 2008 Jan 28.
Recent advances in the identification of mouse plasma cells have enabled a more detailed assessment of their development and maintenance to be undertaken. Insertion of the gene encoding green fluorescent protein into the Blimp1 locus has allowed measurement of the efficiency and kinetics with which subsets of mature B cells generate antibody-secreting cells (ASCs) after culture with a series of mitogens, with and without co-stimulation. In vivo identification of plasma cells has allowed their phenotype to be defined and changes in their frequency as a result of aging and immunisation to be monitored. This new approach has allowed also a more precise definition of the genetic program activated in plasma cell differentiation. In this review we cover these aspects of plasma cell development with a particular emphasis on the B-cell subsets giving rise to the plasma cells and to their maintenance once formed.
小鼠浆细胞鉴定方面的最新进展使得对其发育和维持进行更详细的评估成为可能。将编码绿色荧光蛋白的基因插入Blimp1基因座，使得在有或没有共刺激的情况下，用一系列促细胞分裂剂培养后，能够测量成熟B细胞亚群产生抗体分泌细胞（ASC）的效率和动力学。浆细胞的体内鉴定使得能够定义其表型，并监测衰老和免疫导致的其频率变化。这种新方法还使得能够更精确地定义浆细胞分化过程中激活的基因程序。在本综述中，我们涵盖了浆细胞发育的这些方面，特别强调产生浆细胞的B细胞亚群以及它们形成后的维持。
