Gobbo-Neto Leonardo, Lopes Norberto P
Departamento de Física e Química, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Brazil.
J Agric Food Chem. 2008 Feb 27;56(4):1193-204. doi: 10.1021/jf072812l. Epub 2008 Feb 1.
Lychnophora ericoides Mart. (Asteraceae, Vernonieae) is a plant, endemic to Brazil, with occurrence restricted to the "cerrado" biome. Traditional medicine employs alcoholic and aqueous-alcoholic preparations of leaves from this species for the treatment of wounds, inflammation, and pain. Furthermore, leaves of L. ericoides are also widely used as flavorings for the Brazilian traditional spirit "cachaça". A method has been developed for the extraction and HPLC-DAD analysis of the secondary metabolites of L. ericoides leaves. This analytical method was validated with 11 secondary metabolites chosen to represent the different classes and polarities of secondary metabolites occurring in L. ericoides leaves, and good responses were obtained for each validation parameter analyzed. The same HPLC analytical method was also employed for online secondary metabolite identification by HPLC-DAD-MS and HPLC-DAD-MS/MS, leading to the identification of di- C-glucosylflavones, coumaroylglucosylflavonols, flavone, flavanones, flavonols, chalcones, goyazensolide, and eremantholide-type sesquiterpene lactones and positional isomeric series of chlorogenic acids possessing caffeic and/or ferulic moieties. Among the 52 chromatographic peaks observed, 36 were fully identified and 8 were attributed to compounds belonging to series of caffeoylferuloylquinic and diferuloylquinic acids that could not be individualized from each other.
灯台莲（菊科，斑鸠菊族）是一种原产于巴西的植物，仅生长于“塞拉多”生物群落。传统医学使用该物种叶子的酒精制剂和酒精 - 水混合制剂来治疗伤口、炎症和疼痛。此外，灯台莲的叶子还被广泛用作巴西传统烈酒“卡莎萨”的调味剂。已经开发出一种用于提取和高效液相色谱 - 二极管阵列检测（HPLC - DAD）分析灯台莲叶子次生代谢产物的方法。该分析方法通过选择11种次生代谢产物进行验证，这些次生代谢产物代表了灯台莲叶子中不同类别和极性的次生代谢产物，并且对每个分析的验证参数都获得了良好的响应。相同的HPLC分析方法还用于通过HPLC - DAD - MS和HPLC - DAD - MS/MS进行在线次生代谢产物鉴定，从而鉴定出二 - C - 葡萄糖基黄酮、香豆酰葡萄糖基黄酮醇、黄酮、黄烷酮、黄酮醇、查耳酮、戈亚赞索内酯和艾里曼托内酯型倍半萜内酯以及具有咖啡酸和/或阿魏酸部分的绿原酸的位置异构体系列。在所观察到的52个色谱峰中，36个已被完全鉴定，8个归因于属于咖啡酰阿魏酰奎宁酸和二阿魏酰奎宁酸系列的化合物，这些化合物彼此无法区分。
