Petit T A, Coleman S U, Jones K L, Enright F M, Klei T R
Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Louisiana State University, Baton Rouge 70803.
Exp Parasitol. 1993 Dec;77(4):395-404. doi: 10.1006/expr.1993.1099.
Effects of protective resistance on lymphatic lesions and granulomatous inflammation in infected jirds (Meriones unguiculatus). Experimental Parasitology 77, 395-404. The hypothesis that protective immune responses play a role in the induction of filarial-associated lymphatic lesions was tested in jirds immunized twice with 75 Brugia pahangi radiation-attenuated third-stage larvae. Lymphatic lesions and granulomatous reactivity were compared in immunized, infected, and naive jirds at both acute and chronic periods following challenge with 100 third-stage larvae. Challenge worm burdens were reduced in immunized jirds at both infection periods. The ratio of lymph thrombi to lymphatic worms, an indicator of lymphatic lesion severity, was significantly greater in immunized jirds than in nonimmunized-challenged jirds during acute but not chronic infections. Parasite-specific-granulomatous hypersensitivity was assessed by measurements of granuloma areas around B. pahangi-soluble adult worm antigen-coated sepharose beads embolized in the lungs prior to necropsy. Marked granulomatous inflammatory responses seen during the acute period in both immunized-challenged and nonimmunized-challenged jirds were significantly reduced in similar jirds during chronic periods. Jirds with existing B. pahangi infections were not resistant to homologous challenge infection and had fewer lymphatic lesions and reduced granulomatous inflammatory responses to soluble adult worm antigen compared to previously naive jirds at acute periods postchallenge. These data suggest that protective immune responses increase the severity of filariae-induced lymphatic inflammation. The subsequent modulation of these lesions is probably associated with parasites that survived the protective immune response.
保护性抗性对感染沙鼠（长爪沙鼠）淋巴病变和肉芽肿性炎症的影响。《实验寄生虫学》77卷，395 - 404页。用75条辐射减毒的彭亨布鲁线虫第三期幼虫对沙鼠进行两次免疫，以检验保护性免疫反应在丝虫相关淋巴病变诱导中起作用这一假说。在用100条第三期幼虫攻击后的急性期和慢性期，对免疫、感染和未感染的沙鼠的淋巴病变和肉芽肿反应性进行了比较。在两个感染期，免疫沙鼠的攻击虫负荷均降低。淋巴血栓与淋巴线虫的比例是淋巴病变严重程度的一个指标，在急性期，免疫沙鼠的这一比例显著高于未免疫攻击的沙鼠，但在慢性感染期则不然。在尸检前，通过测量肺中包被彭亨布鲁线虫可溶性成虫抗原的琼脂糖珠周围的肉芽肿面积来评估寄生虫特异性肉芽肿超敏反应。在急性期，免疫攻击和未免疫攻击的沙鼠中均可见明显的肉芽肿性炎症反应，而在慢性期，类似沙鼠中的这种反应显著降低。与攻击后急性期之前未感染过的沙鼠相比，已感染彭亨布鲁线虫的沙鼠对同源攻击感染没有抗性，且淋巴病变较少，对可溶性成虫抗原的肉芽肿性炎症反应也有所降低。这些数据表明，保护性免疫反应会增加丝虫诱导的淋巴炎症的严重程度。这些病变随后的调节可能与在保护性免疫反应中存活下来的寄生虫有关。
