Carapito Raphaël, Hatsch Didier, Vorwerk Sonja, Petkovski Elizabet, Jeltsch Jean-Marc, Phalip Vincent
U.M.R. 7175, Université Louis Pasteur-CNRS, Ecole Supérieure de Biotechnologie de Strasbourg, Boulevard Sébastien Brant, BP 10413, 67412 Illkirch-Graffenstaden, France.
Fungal Genet Biol. 2008 May;45(5):738-48. doi: 10.1016/j.fgb.2007.12.002. Epub 2007 Dec 10.
Fusarium graminearum is a phytopathogenic filamentous fungus attacking a wide range of plants including Humulus lupulus (hop). Transcriptional analysis of F. graminearum grown on minimal media containing hop cell wall or glucose as the sole carbon source was performed by applying a highly stringent method combining microarrays and a subtracted cDNA library. In addition to genes coding for various cell wall degrading enzymes (CWDE), several metabolic pathways were induced in response to the plant cell wall substrate. Many genes participating in these pathways are probably involved in cellular transport. But the most interesting was that all the genes composing the 4-aminobutyrate-shunt (GABA-shunt) were also up-regulated in the presence of plant cell wall material and were present in the cDNA library. This study provides a description of a part of the fungal gene expression profile when it is in contact with raw biological materials, and helps in understanding the plant cell wall degradation and the infection process.
禾谷镰刀菌是一种植物病原丝状真菌，可侵袭包括啤酒花（Humulus lupulus）在内的多种植物。通过应用一种结合微阵列和消减cDNA文库的高度严格方法，对在含有啤酒花细胞壁或葡萄糖作为唯一碳源的基本培养基上生长的禾谷镰刀菌进行了转录分析。除了编码各种细胞壁降解酶（CWDE）的基因外，响应植物细胞壁底物还诱导了几种代谢途径。参与这些途径的许多基因可能与细胞运输有关。但最有趣的是，构成4-氨基丁酸分流（GABA分流）的所有基因在存在植物细胞壁材料时也被上调，并且存在于cDNA文库中。这项研究描述了真菌与天然生物材料接触时部分基因表达谱，有助于理解植物细胞壁降解和感染过程。
