National Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
Mycotoxin Res. 2013 May;29(2):103-11. doi: 10.1007/s12550-013-0158-y. Epub 2013 Jan 31.
The metabolic products resulting from the cultivation of F. asiaticum in agmatine were identified using capillary electrophoresis-time of flight mass spectrometry. Glyoxylic acid was detected from fungal cultures grown in agmatine, while it was absent in control cells. The abundance of other metabolic products of the glycolytic pathway also increased because of agmatine; however, there was no increase in the amounts of pyruvic acid or metabolites from the tricarboxylic acid cycle. Moreover, gene expression levels within Fusarium asiaticum exposed to agmatine were analyzed by DNA microarray. Changes in gene expression levels directed the changes in metabolic products. Our results suggest that acetyl coenzyme A, which is a starting substrate for the biosynthesis of deoxynivalenol (DON), was simultaneously produced by activated β-oxidation. Furthermore, the content of 4-aminobutyrate (GABA) was increased in the agmatine addition culture medium. GABA can be synthesized from agmatine through putrescine and might influence the regulation of DON-related genes.
采用毛细管电泳-飞行时间质谱法鉴定了在胍丁胺中培养的亚洲镰刀菌的代谢产物。在胍丁胺中生长的真菌培养物中检测到乙醛酸，而在对照细胞中则没有。由于胍丁胺的存在，糖酵解途径的其他代谢产物的丰度也增加了；然而，丙酮酸或三羧酸循环的代谢物的量没有增加。此外，还通过 DNA 微阵列分析了暴露于胍丁胺的亚洲镰刀菌的基因表达水平。基因表达水平的变化导致代谢产物的变化。我们的结果表明，乙酰辅酶 A 是脱氧雪腐镰刀菌烯醇 (DON) 生物合成的起始底物，同时通过激活β-氧化产生。此外，在胍丁胺添加培养基中，4-氨基丁酸 (GABA) 的含量增加。GABA 可以通过腐胺从胍丁胺合成，可能影响 DON 相关基因的调控。
