Fawzy Shereen, Sallam Maha, Awad Nahla Mohammad
Medical Microbiology and Immunology Department, Faculty of Medicine Ain Shams University, Abbassia, Cairo, Egypt.
Clin Biochem. 2008 May;41(7-8):486-92. doi: 10.1016/j.clinbiochem.2007.12.017. Epub 2008 Jan 3.
The association of oncogenic EBV with breast carcinoma (BC) is still in controversy.
Assess the association of EBV with BC in Egyptian women and find possible relationship between prognostic factors of BC and EBV detection.
Paraffin-embedded sections from 40 female patients with primary invasive BC; ductal (n=32) and lobular (n=8) and breast tissues from patients with fibrocystic disease (n=20) as control were screened for presence of EBV by EBV nuclear antigen-1 (EBNA-1) immunostaining and by PCR for EBV-DNA.
10/40 (25%) of the BC specimens stained positively for EBNA-1; EBNA-1 expression was restricted to a fraction 5%-60% of tumor epithelial cells. EBV-DNA was detected in 8/10 of BC specimens positive for EBNA-1. Control specimens were negative by both techniques. 7/8 (87.5%) of EBV-DNA positive tumors were associated with >3 lymph nodes involvement.
EBV is associated with some invasive BC in Egyptian females and may play a role in their etiology.
致癌性EB病毒与乳腺癌（BC）之间的关联仍存在争议。
评估埃及女性中EB病毒与乳腺癌的关联，并找出乳腺癌预后因素与EB病毒检测之间的可能关系。
对40例原发性浸润性乳腺癌女性患者（导管癌32例，小叶癌8例）以及20例纤维囊性疾病患者的乳腺组织（作为对照）的石蜡包埋切片，通过EB病毒核抗原-1（EBNA-1）免疫染色和EB病毒DNA的聚合酶链反应（PCR）来筛查EB病毒的存在情况。
40例乳腺癌标本中有10例（25%）EBNA-1染色呈阳性；EBNA-1表达局限于肿瘤上皮细胞的5%至60%。在EBNA-1阳性的10例乳腺癌标本中有8例检测到EB病毒DNA。两种技术检测对照标本均为阴性。8例EB病毒DNA阳性肿瘤中有7例（87.5%）与3个以上淋巴结受累相关。
EB病毒与埃及女性的某些浸润性乳腺癌相关，可能在其病因学中起作用。
