Hamady Micah, Walker Jeffrey J, Harris J Kirk, Gold Nicholas J, Knight Rob
Department of Computer Science, UCB 430, University of Colorado, Boulder, Colorado 80309, USA.
Nat Methods. 2008 Mar;5(3):235-7. doi: 10.1038/nmeth.1184. Epub 2008 Feb 10.
We constructed error-correcting DNA barcodes that allow one run of a massively parallel pyrosequencer to process up to 1,544 samples simultaneously. Using these barcodes we processed bacterial 16S rRNA gene sequences representing microbial communities in 286 environmental samples, corrected 92% of sample assignment errors, and thus characterized nearly as many 16S rRNA genes as have been sequenced to date by Sanger sequencing.
我们构建了纠错DNA条形码,使得一次大规模平行焦磷酸测序能够同时处理多达1544个样本。利用这些条形码,我们处理了代表286个环境样本中微生物群落的细菌16S rRNA基因序列,校正了92%的样本分配错误,因此对16S rRNA基因的表征数量几乎与迄今为止通过桑格测序法测序得到的数量相当。