来自大肠杆菌23S核糖体RNA的69号螺旋的pH依赖性结构变化。
pH-dependent structural changes of helix 69 from Escherichia coli 23S ribosomal RNA.
作者信息
Abeysirigunawardena Sanjaya C, Chow Christine S
机构信息
Department of Chemistry, Wayne State University, Detroit, Michigan 48202, USA.
出版信息
RNA. 2008 Apr;14(4):782-92. doi: 10.1261/rna.779908. Epub 2008 Feb 11.
Abstract
Helix 69 in 23S rRNA is a region in the ribosome that participates in a considerable number of RNA-RNA and RNA-protein interactions. Conformational flexibility is essential for such a region to interact and accommodate protein factors at different stages of protein biosynthesis. In this study, pH-dependent structural and stability changes were observed for helix 69 through a variety of spectroscopic techniques, such as circular dichroism spectroscopy, UV melting, and nuclear magnetic resonance spectroscopy. In Escherichia coli 23S rRNA, helix 69 contains pseudouridine residues at positions 1911, 1915, and 1917. The presence of these pseudouridines was found to be essential for the pH-induced conformational changes. Some of the pH-dependent changes appear to be localized to the loop region of helix 69, emphasizing the importance of the highly conserved nature of residues in this region.
摘要
23S核糖体RNA中的螺旋69是核糖体中的一个区域，它参与了大量的RNA-RNA和RNA-蛋白质相互作用。构象灵活性对于该区域在蛋白质生物合成的不同阶段与蛋白质因子相互作用并容纳它们至关重要。在本研究中，通过多种光谱技术，如圆二色光谱、紫外熔解和核磁共振光谱，观察到了螺旋69的pH依赖性结构和稳定性变化。在大肠杆菌23S核糖体RNA中，螺旋69在1911、1915和1917位含有假尿苷残基。发现这些假尿苷的存在对于pH诱导的构象变化至关重要。一些pH依赖性变化似乎局限于螺旋69的环区域，强调了该区域残基高度保守性质的重要性。
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