糖皮质激素对膜联蛋白I的蛋白质合成依赖性诱导

Protein-synthesis-dependent induction of annexin I by glucocorticoid.

作者信息

Wong W T, Frost S C, Nick H S

机构信息

Department of Biochemistry and Molecular Biology, College of Medicine, University of Florida, Gainesville 32610.

出版信息

Biochem J. 1991 Apr 15;275 ( Pt 2)(Pt 2):313-9. doi: 10.1042/bj2750313.

DOI:10.1042/bj2750313

PMID:1827255

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1150054/

Abstract

We demonstrate that annexin I/lipocortin I (lipo I) gene expression is regulated by dexamethasone (DEX) in mouse 3T3-L1 fibroblasts and LA-4 lung epithelial cells. We have characterized this induction further in 3T3-L1 fibroblasts. At 24 h after addition of DEX, the levels of lipo I mRNA and protein increased 5-fold and 1.5-fold respectively. Time-course experiments revealed that the induction was delayed by 2-4 h after DEX addition. Half-maximal induction of both lipo I mRNA and protein was achieved with 10 nM-DEX. Both actinomycin D and cycloheximide blocked the DEX effect on lipo I mRNA expression. These results indicate that the induction of lipo I by DEX has a transcriptional component and requires protein synthesis de novo.

摘要

我们证明，在小鼠3T3-L1成纤维细胞和LA-4肺上皮细胞中，膜联蛋白I/脂皮质蛋白I（脂皮质蛋白I）基因表达受地塞米松（DEX）调控。我们在3T3-L1成纤维细胞中进一步对这种诱导作用进行了表征。添加DEX后24小时，脂皮质蛋白I mRNA和蛋白质水平分别增加了5倍和1.5倍。时间进程实验表明，添加DEX后诱导作用延迟2-4小时。10 nM DEX可实现脂皮质蛋白I mRNA和蛋白质的半数最大诱导。放线菌素D和环己酰亚胺均阻断了DEX对脂皮质蛋白I mRNA表达的作用。这些结果表明，DEX对脂皮质蛋白I的诱导具有转录成分，并且需要从头合成蛋白质。