Reviglio Victor E, Sambuelli Ruben H, Olmedo Alejandra, Falco Micaela, Echenique Jose, O'Brien Terrence P, Kuo Irene C
Cornea and External Eye Diseases Lab, School of Medicine, Catholic University of Cordoba, Cordoba 5021, Argentina.
Mediators Inflamm. 2007;2007:93857. doi: 10.1155/2007/93857.
To describe the presence of secretory leukocyte protease inhibitor (SLPI), a cationic peptide with antimicrobial and antiprotease activity, in the innate ocular immune reaction in a rat model of Staphylococcus aureus endophthalmitis.
Seventy-five female Lewis rats were divided into three groups: the endophthalmitis group received an intravitreal injection of 65 colony-forming units of viable S. aureus, the vehicle-injected group received balanced sterile saline solution (BSS), and the control group was not injected. Eyes were enucleated at 24 and 48 hours and processed for immunohistochemical staining and Western blot studies for SLPI.
In S. aureus endophthalmitis eyes, there was strong immunostaining for SLPI in the retina and vitreous with associated neutrophilic infiltrates. At 48 hours, corneas also stained for SLPI. Western blots confirmed increased SLPI expression in all infected eyes. By immunohistochemical assays, SLPI was absent in the BSS and control eyes. The causative pathogen was identified in all samples from the endophthalmitis group by traditional culture methods.
To our knowledge, this report is the first to demonstrate the presence of SLPI in the inflamed cornea, vitreous, and retina tissues of rat eyes with S. aureus endophthalmitis, suggesting that SLPI has an active role in the innate immunity of the eye. Release of SLPI by inflammatory cells in the anterior and posterior segments may contribute to the host defense response against infectious endophthalmitis.
描述分泌型白细胞蛋白酶抑制剂（SLPI），一种具有抗菌和抗蛋白酶活性的阳离子肽，在金黄色葡萄球菌性眼内炎大鼠模型的先天性眼部免疫反应中的存在情况。
75只雌性Lewis大鼠被分为三组：眼内炎组接受玻璃体内注射65个菌落形成单位的活金黄色葡萄球菌，注射赋形剂组接受平衡无菌盐溶液（BSS），对照组不进行注射。在24小时和48小时摘除眼球，进行免疫组织化学染色和针对SLPI的蛋白质印迹研究。
在金黄色葡萄球菌性眼内炎的眼中，视网膜和玻璃体中SLPI有强烈免疫染色，伴有嗜中性粒细胞浸润。在48小时时，角膜也对SLPI染色。蛋白质印迹证实所有感染眼的SLPI表达增加。通过免疫组织化学分析，BSS组和对照组眼中不存在SLPI。通过传统培养方法在眼内炎组的所有样本中鉴定出致病病原体。
据我们所知，本报告首次证明在患有金黄色葡萄球菌性眼内炎的大鼠眼的炎症角膜、玻璃体和视网膜组织中存在SLPI，表明SLPI在眼部先天性免疫中起积极作用。前节和后节炎症细胞释放SLPI可能有助于宿主对感染性眼内炎的防御反应。
