Kang Yingqian, Iida Soji, Yamamoto Setsuya, Kogure Takahisa, Tanaka Reiko, Mikami Yuzuru
Medical Mycology Research Center, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba, Japan.
Nihon Ishinkin Gakkai Zasshi. 2008;49(1):39-43. doi: 10.3314/jjmm.49.39.
Comparative studies of random amplified polymorphic DNA (RAPD) band patterns of Candida tropicalis with those of clinically important Candida species have shown the presence of specific RAPD bands for C. tropicalis. A band specific to C. tropicalis strains (ca. 400 bp) was extracted and sequenced. It was found to belong to a fragment of the Trf4 gene, which is essential for growth of these strains and has a characteristic sequence of C. tropicalis. A PCR primer was designed specifically for C. tropicalis which amplifies the 324 bp band. The PCR primer amplified DNA products for all C. tropicalis strains tested, but did not amplify any PCR bands from C. albicans, C. dubliniensis, C. glabrata, C. guilliermondii, C. kefer, C. krusei, C. parapsilosis, or C. zeylanoides. Usefulness of the PCR primer in differentiating from clinical isolates of other fungal species is discussed.
热带假丝酵母菌与临床上重要的念珠菌属菌种的随机扩增多态性DNA（RAPD）条带模式的比较研究表明，热带假丝酵母菌存在特定的RAPD条带。提取并测序了一条热带假丝酵母菌菌株特有的条带（约400 bp）。发现它属于Trf4基因的一个片段，该基因对这些菌株的生长至关重要，并且具有热带假丝酵母菌的特征序列。专门为热带假丝酵母菌设计了一个PCR引物，该引物可扩增出324 bp的条带。该PCR引物能扩增出所有测试的热带假丝酵母菌菌株的DNA产物，但不能从白色念珠菌、都柏林念珠菌、光滑念珠菌、季也蒙念珠菌、凯弗念珠菌、克鲁斯念珠菌、近平滑念珠菌或锡兰念珠菌中扩增出任何PCR条带。讨论了该PCR引物在区分其他真菌临床分离株方面的实用性。
