Guarino H, Núñez A, Repiso M V, Gil A, Dargatz D A
Ministry of Livestock, Agriculture and Fisheries, Veterinary Laboratories Division, Miguel C. Rubino, Ruta 8 km 17.500, P.O. Box 6577, Montevideo, Uruguay.
Prev Vet Med. 2008 Jun 15;85(1-2):34-40. doi: 10.1016/j.prevetmed.2007.12.012. Epub 2008 Feb 15.
Our objective was to determine the prevalence of serum antibodies to bovine herpesvirus-1 (BHV-1) and bovine viral diarrhea (BVD) virus in beef cattle in Uruguay. A random sample of 230 herds selected with probability proportional to population size based on the number of cattle was chosen from a list frame of all registered livestock farms as of June 1999. Sera from up to 10 heifers, cows and bulls (up to 30 sera total per herd) were collected on selected farms between March 2000 and March 2001 and evaluated by means of enzyme-linked immunosorbent assays (ELISAs). Overall, 6358 serum samples were evaluated. We also collected data on previous diagnosis of BHV-1 or BVD infections and on the use of vaccines against these agents. The estimated prevalence of exposure to BHV-1 and BVD at the herd level for the Uruguayan beef population was 99% and 100%, respectively. Approximately 37% of beef cattle in Uruguay have been exposed to BHV-1 and 69% to BVD virus. Only 3% of beef herds in Uruguay regularly (typically, annually) use vaccines against either of these agents.
我们的目标是确定乌拉圭肉牛中牛疱疹病毒1型（BHV - 1）和牛病毒性腹泻（BVD）病毒血清抗体的流行情况。根据牛的数量，从截至1999年6月所有注册畜牧场的列表框架中，选取了230个与种群规模成概率比例的随机样本牛群。在2000年3月至2001年3月期间，从选定的农场收集了多达10头小母牛、母牛和公牛的血清（每个牛群最多30份血清），并通过酶联免疫吸附测定（ELISA）进行评估。总体而言，共评估了6358份血清样本。我们还收集了有关BHV - 1或BVD感染既往诊断情况以及针对这些病原体的疫苗使用情况的数据。乌拉圭肉牛群体中，估计牛群层面BHV - 1和BVD的暴露流行率分别为99%和100%。乌拉圭约37%的肉牛曾接触过BHV - 1，69%曾接触过BVD病毒。乌拉圭只有3%的肉牛群定期（通常为每年）使用针对这两种病原体之一的疫苗。
