Poole Leslie B, Nelson Kimberly J
Department of Biochemistry, Center for Structural Biology, Wake Forest University School of Medicine, Winston-Salem, NC 27157, United States.
Curr Opin Chem Biol. 2008 Feb;12(1):18-24. doi: 10.1016/j.cbpa.2008.01.021. Epub 2008 Mar 7.
Accumulating evidence reveals hydrogen peroxide as a key player both as a damaging agent and, from emerging evidence over the past decade, as a second messenger in intracellular signaling. This rather mild oxidant acts upon downstream targets within signaling cascades to modulate the activity of a host of enzymes (e.g. phosphatases and kinases) and transcriptional regulators through chemoselective oxidation of cysteine residues. With the recent development of specific detection reagents for hydrogen peroxide and new chemical tools to detect the generation of the initial oxidation product, sulfenic acid, on reactive cysteines within target proteins, the scene is set to gain a better understanding of the mechanisms through which hydrogen peroxide acts as a second messenger in cell signaling.
越来越多的证据表明，过氧化氢既是一种破坏剂，也是一种细胞内信号传导的第二信使（基于过去十年新出现的证据）。这种相对温和的氧化剂作用于信号级联反应中的下游靶点，通过对半胱氨酸残基进行化学选择性氧化来调节许多酶（如磷酸酶和激酶）和转录调节因子的活性。随着过氧化氢特异性检测试剂以及用于检测目标蛋白中反应性半胱氨酸上初始氧化产物亚磺酸生成的新化学工具的最新发展，我们有望更好地理解过氧化氢在细胞信号传导中作为第二信使的作用机制。
