Ren Zhihong, Guo Zhuyan, Meydani Simin Nikbin, Wu Dayong
Nutritional Immunology Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, Boston, MA 02111, USA.
J Nutr. 2008 Mar;138(3):544-50. doi: 10.1093/jn/138.3.544.
Mushrooms have been shown to enhance immune response, which contributes to their antitumor property. White button mushrooms (Agaricus bisporus) (WBM) constitute 90% of the total mushrooms consumed in the United States; however, the health benefit of this strain in general is not well studied. Furthermore, little is known about WBM's immunologic effects. Dendritic cells (DC) are the most potent antigen presenting cells and play a pivotal role in immune response by linking innate and adaptive immune responses. In this study, we investigated the effect of in vitro supplementation with WBM on maturation of bone marrow-derived DC (BMDC) of C57BL mice. BMDC were differentiated in the presence of whole mushroom concentrate at 50, 100, or 200 mg/L. Results showed that mushroom supplementation dose dependently increased the expression of maturation markers CD40, CD80, CD86, and major histocompatibility complex-II. Consistent with the changes in the phenotypic markers, functional assay for DC maturation showed that mushroom supplementation decreased DC endocytosis and increased intracellular interleukin (IL)-12 levels. Furthermore, using a syngeneic T cell activation model, we found that WBM-supplemented DC from BALB/c mice presented ovalbumin antigen to T cells from DO11.10 mice more efficiently as demonstrated by increased T cell proliferation and IL-2 production. In conclusion, WBM promote DC maturation and enhance their antigen-presenting function. This effect may have potential in enhancing both innate and T cell-mediated immunity leading to a more efficient surveillance and defense mechanism against microbial invasion and tumor development.
蘑菇已被证明可增强免疫反应，这有助于其抗肿瘤特性。白蘑菇（双孢蘑菇）在美国消费的蘑菇总量中占90%；然而，该菌株的总体健康益处尚未得到充分研究。此外，关于白蘑菇的免疫作用知之甚少。树突状细胞（DC）是最有效的抗原呈递细胞，通过连接先天免疫和适应性免疫反应在免疫反应中起关键作用。在本研究中，我们调查了体外补充白蘑菇对C57BL小鼠骨髓来源的DC（BMDC）成熟的影响。BMDC在存在50、100或200mg/L的全蘑菇浓缩物的情况下进行分化。结果表明，蘑菇补充剂剂量依赖性地增加了成熟标志物CD40、CD80、CD86和主要组织相容性复合体-II的表达。与表型标志物的变化一致，DC成熟的功能测定表明，蘑菇补充剂降低了DC内吞作用并增加了细胞内白细胞介素（IL）-12水平。此外, 使用同基因T细胞激活模型，我们发现来自BALB/c小鼠的补充白蘑菇的DC更有效地将卵清蛋白抗原呈递给来自DO11.10小鼠的T细胞，这表现为T细胞增殖和IL-2产生增加。总之，白蘑菇促进DC成熟并增强其抗原呈递功能。这种作用可能在增强先天免疫和T细胞介导的免疫方面具有潜力，从而导致对微生物入侵和肿瘤发展的更有效的监测和防御机制。
