Gurbuz Taskin, Ozdemir Yuksel, Kara Nihal, Zehir Cigdem, Kurudirek Murat
Department of Pediatric Dentistry, Faculty of Dentistry, Ataturk University, Erzurum-Turkey.
J Endod. 2008 Mar;34(3):318-21. doi: 10.1016/j.joen.2007.12.016.
This study evaluated in vitro any changes in the morphology and mineral content of root canal dentin after treatments with Nd:YAG laser irradiation and 5 different irrigation solutions. The groups of extracted single-rooted human teeth were exposed to sterile saline solution, 5.25% NaOCl, 3% H2O2, 15% ethylenediaminetetraacetic acid, 2% chlorhexidine gluconate, and Nd:YAG laser irradiation. All prepared teeth were bisected longitudinally; half of each tooth was used for wavelength dispersive x-ray fluorescence spectrometry analysis to assess the mineral content. The other half was studied by using scanning electron microscopy to analyze the surface of the dentin. The results showed that the calcium level and calcium/phosphorus ratio decreased significantly with the NaOCl irrigation solutions (p < .05), suggesting the presence of changes at molecular level. Significant differences among the test groups were also observed in the scanning electron microscopy evaluation (p < .05). The lowest scores for root cleaning were obtained by using ethylenediaminetetraacetic acid followed by Nd:YAG laser treatment.
本研究评估了用钕钇铝石榴石（Nd:YAG）激光照射和5种不同冲洗液处理后根管牙本质的形态和矿物质含量的体外变化。将拔除的单根人牙分组，分别暴露于无菌盐溶液、5.25%次氯酸钠（NaOCl）、3%过氧化氢（H2O2）、15%乙二胺四乙酸（EDTA）、2%葡萄糖酸氯己定以及Nd:YAG激光照射下。所有制备好的牙齿均沿纵向一分为二；每颗牙齿的一半用于波长色散X射线荧光光谱分析以评估矿物质含量。另一半则通过扫描电子显微镜研究以分析牙本质表面。结果显示，使用NaOCl冲洗液后钙水平和钙/磷比值显著降低（p < 0.05），表明在分子水平存在变化。在扫描电子显微镜评估中，各测试组之间也观察到显著差异（p < 0.05）。使用乙二胺四乙酸后再进行Nd:YAG激光处理，根管清洁得分最低。
