Brewster J A, Orsi N M, Gopichandran N, Ekbote U V, Cadogan E, Walker J J
Perinatal Research Group, The Liz Dawn Pathology and Translational Sciences Centre, Level 4, Leeds Institute of Molecular Medicine, JIF Building, St James's University Hospital, Leeds, UK.
Hypertens Pregnancy. 2008;27(1):1-16. doi: 10.1080/10641950701826067.
The pathophysiology of preeclampsia (PET) implicates an inflammatory dysfunction. This study profiled this host response by challenging whole blood with lipopolysaccharide. Multiplex immunoassays determined interleukin (IL)-1beta, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12(p70), IL-13, IL-17, granulocyte/granulocyte macrophage-colony stimulating factors (G-CSF/GM-SCF), interferon(IFN)-gamma, monocyte chemotactic protein (MCP)-1, macrophage inflammatory protein-1beta and tumor necrosis factor (TNF)-alpha levels. Secretory capacity was expressed in pg/million white cells or monocytes (+/-SEM). PET featured significantly higher IL-1beta, IL-2, IL-10, IL-13, G-CSF, IFN-gamma, MCP-1 and TNF-alpha monocyte secretory capacities (p < 0.05). The PET group exhibited an inflammatory hyper-responsiveness (p < 0.01) which was poorly described by the traditional Th1:Th2 dichotomy.
子痫前期（PET）的病理生理学涉及炎症功能障碍。本研究通过用脂多糖刺激全血来分析这种宿主反应。多重免疫测定法测定白细胞介素（IL）-1β、IL-2、IL-4、IL-5、IL-6、IL-7、IL-8、IL-10、IL-12（p70）、IL-13、IL-17、粒细胞/粒细胞巨噬细胞集落刺激因子（G-CSF/GM-SCF）、干扰素（IFN）-γ、单核细胞趋化蛋白（MCP）-1、巨噬细胞炎性蛋白-1β和肿瘤坏死因子（TNF）-α水平。分泌能力以每百万白细胞或单核细胞的皮克数表示（±标准误）。PET的特点是IL-1β、IL-2、IL-10、IL-13、G-CSF、IFN-γ、MCP-1和TNF-α单核细胞分泌能力显著更高（p<0.05）。PET组表现出炎症高反应性（p<0.01），传统的Th1:Th2二分法对此描述不佳。
