Department of Chemistry, Institute of Biological Sciences, Faculty of Medicine, the John Paul II Catholic University of Lublin, Konstantynów 1J, 20-708, Lublin, Poland.
Department of Polymer Chemistry, Faculty of Chemistry, Maria Curie-Skłodowska University, Gliniana 33, 20-614, Lublin, Poland.
Sci Rep. 2024 Aug 11;14(1):18622. doi: 10.1038/s41598-024-69491-4.
Tryptophan (an essential amino acid) and its clinically important metabolite-kynurenine contribute to several fundamental biological processes and methods that allow their determination in biological samples are in demand. The novelty of the work was a demonstration of the utility of two polymers: 4-vinylpyridine crosslinked with trimethylolpropane trimethacrylate (poly(4VP-co-TRIM)) or 1,4-dimethacryloyloxybenzene (poly(4VP-co-14DMB))-in terms of human serum clean-up for simultaneous LC-MS determination of tryptophan and kynurenine. The goal was to achieve a reduction of the matrix effect, which is responsible for signal suppression, with minimal capture of analytes. The adsorption properties of the polymeric beads were studied by evaluating the adsorption kinetics and isotherms in model matrices. Therefore, the adsorption capacities of both molecules were not efficient, the tested 4-vinylpyridine-based copolymers have shown great promise (especially poly(4VP-co-TRIM)) as sorbents for serum clean-up. In the model human serum matrix, poly(4VP-co-TRIM) provided good recoveries of tryptophan and kynurenine (76% and 87%, respectively) and allowed for the reduction of the matrix effect. Performances of both copolymers were compared to those of commercially available sorbents (octadecylsilane, activated charcoal, and primary secondary amine).
色氨酸（一种必需氨基酸）及其具有临床重要意义的代谢产物犬尿氨酸参与了几种基本的生物学过程，因此对其在生物样本中的测定方法的需求也在不断增加。这项工作的新颖之处在于展示了两种聚合物的实用性：与三羟甲基丙烷三甲基丙烯酸酯交联的 4-乙烯基吡啶（聚（4VP-co-TRIM））或 1,4-二甲丙烯酰氧基苯（聚（4VP-co-14DMB））-在用于色氨酸和犬尿氨酸同时 LC-MS 测定的人血清净化方面。目的是实现减少基质效应，基质效应会导致信号抑制，同时尽量减少分析物的捕获。通过评估模型基质中的吸附动力学和等温线来研究聚合物珠的吸附特性。因此，两种分子的吸附容量都不高，所测试的基于 4-乙烯基吡啶的共聚物显示出很大的潜力（特别是聚（4VP-co-TRIM））作为血清净化的吸附剂。在模型人血清基质中，聚（4VP-co-TRIM）对色氨酸和犬尿氨酸的回收率良好（分别为 76%和 87%），并能降低基质效应。两种共聚物的性能与市售的吸附剂（十八烷基硅烷、活性炭和仲伯胺）进行了比较。
