2016 - 2017年中国广东省西部花生油中的黄曲霉毒素B

Aflatoxin B in peanut oil from Western Guangdong, China, during 2016-2017.

作者信息

Qi Ningli, Yu Hailong, Yang Chunliang, Gong Xiao, Liu Yangyang, Zhu Yiting

机构信息

a Chinese Academy of Tropical Agricultural Sciences Center for Food Quality Supervision and Testing Ministry of Agriculture (Zhanjiang), Agricultural Product Processing Research Institute , Chinese Academy of Tropical Agricultural Sciences , Zhanjiang , China.

b Institute of Quality Standard and Testing Technology For Agro-Products , Chinese Academy of Agricultural Sciences , Beijing , China.

出版信息

Food Addit Contam Part B Surveill. 2019 Mar;12(1):45-51. doi: 10.1080/19393210.2018.1544173. Epub 2018 Nov 18.

DOI:10.1080/19393210.2018.1544173

PMID:30451088

Abstract

Aflatoxin B (AFB) occurrence in peanut oil samples randomly collected from family workshops in western Guangdong during 2016-2017 (n = 427) was surveyed. AFB content was screened by enzyme-linked immunosorbent assay (ELISA) protocol and analytically confirmed with an UPLC-MS/MS method. The limit of detection of the ELISA method was 1.08 μg kg. The recovery values ranged from 84.4 to 92.6% with relative standard deviations of 2.2 to 4.8%. AFB was quantified in 47 samples (22.5%) with a range of 15.4-49.9 μg kg in 2016 and in 33 samples (15.1%) with 8.8-22.2 μg kg in 2017, respectively. The AFB contamination in peanut oil was season-depended in western Guangdong with the worst case in spring (24.2-37.9%). Overall, a significant reduction of AFB occurrence was observed in western Guangdong after 2016. It is advisable to control AFB in bulk and self-pressed oil from family workshops and regulate it mandatorily if necessary.

摘要

对2016 - 2017年期间从粤西家庭作坊随机采集的花生油样本（n = 427）中黄曲霉毒素B（AFB）的存在情况进行了调查。通过酶联免疫吸附测定（ELISA）方法筛选AFB含量，并采用超高效液相色谱 - 串联质谱法（UPLC-MS/MS）进行分析确认。ELISA方法的检测限为1.08 μg/kg。回收率在84.4%至92.6%之间，相对标准偏差为2.2%至4.8%。2016年，在47个样本（22.5%）中检测到AFB，含量范围为15.4 - 49.9 μg/kg；2017年，在33个样本（15.1%）中检测到AFB，含量范围为8.8 - 22.2 μg/kg。粤西花生油中的AFB污染与季节有关，春季污染情况最严重（24.2% - 37.9%）。总体而言，2016年后粤西AFB的发生率显著降低。建议对家庭作坊生产的散装油和自榨油中的AFB进行控制，必要时进行强制监管。