Gornik Olga, Gornik Ivan, Gasparović Vladimir, Lauc Gordan
Department of Biochemistry and Molecular Biology, University of Zagreb, Faculty of Pharmacy and Biochemistry, Ante Kovacića 1, Zagreb, Croatia.
Clin Biochem. 2008 May;41(7-8):504-10. doi: 10.1016/j.clinbiochem.2008.01.026. Epub 2008 Feb 15.
Early prediction of severe acute pancreatitis is one of the problems in clinical practice. Since many diseases are associated with alteration in glycosylation, in this work we studied sialylation of transferrin and serum proteins in acute pancreatitis.
Sialylation was analyzed during first eight days of hospitalization of 30 patients and compared to 28 healthy controls. Transferrin sialylation was measured using enzyme linked lectin assay, while sialic acid on proteins was measured using resorcinol method.
Both analyzed parameters changed during studied period. The change in transferrin sialylation between Day1 and Day2 of hospitalization was shown to be an early prognostic marker of acute pancreatitis, with better sensitivity (88.9%) and specificity (90.5%) than other markers tested.
Sialylation of transferrin and total serum proteins reflects the intensity of inflammatory response during acute pancreatitis and could be used as prognostic parameter for disease severity.
早期预测重症急性胰腺炎是临床实践中的难题之一。由于许多疾病都与糖基化改变有关，因此在本研究中，我们对急性胰腺炎患者转铁蛋白和血清蛋白的唾液酸化进行了研究。
分析了30例患者住院头八天的唾液酸化情况，并与28名健康对照者进行比较。采用酶联凝集素测定法检测转铁蛋白的唾液酸化，采用间苯二酚法检测蛋白质上的唾液酸。
在研究期间，两个分析参数均发生了变化。住院第1天和第2天转铁蛋白唾液酸化的变化被证明是急性胰腺炎的早期预后标志物，其敏感性（88.9%）和特异性（90.5%）优于其他检测标志物。
转铁蛋白和总血清蛋白的唾液酸化反映了急性胰腺炎期间炎症反应的强度，可作为疾病严重程度的预后参数。
