Muñoz-Berbel X, Vigués N, Jenkins A T A, Mas J, Muñoz F J
Centro Nacional de Microelectrónica (IMB-CSIC), Esfera UAB, Campus Univ. Autónoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.
Biosens Bioelectron. 2008 May 15;23(10):1540-6. doi: 10.1016/j.bios.2008.01.007. Epub 2008 Jan 17.
This paper describes an approach for quantifying low concentrations of bacteria, particularly Escherichia coli, based on the measurement of the initial attachment of bacteria to platinum surfaces, using impedance spectroscopy. The value of the interface capacitance in the pre-attachment stage (before 1min of attachment) showed correlation with suspended concentration of bacteria from 10(1) to 10(7)CFUmL(-1) (colony forming units per mL). This method was found to be sensitive to the attachment time, to the applied potential and to the size of the counter electrode. The sensor lifetime was also evaluated.
本文描述了一种基于使用阻抗谱测量细菌在铂表面的初始附着情况来定量低浓度细菌，特别是大肠杆菌的方法。附着前阶段（附着1分钟之前）的界面电容值与10¹至10⁷CFUmL⁻¹（每毫升菌落形成单位）的细菌悬浮浓度相关。发现该方法对附着时间、施加的电位和对电极的尺寸敏感。还评估了传感器的寿命。
