Franke Adrian A, Halm Brunhild M, Ashburn Leslie A
Cancer Research Center of Hawai'i, Natural Products & Cancer Biology Program, 1236 Lauhala Street, Honolulu, HI 96813, USA.
Arch Biochem Biophys. 2008 Aug 15;476(2):161-70. doi: 10.1016/j.abb.2008.02.009. Epub 2008 Feb 14.
Soy and their isoflavones (IFLs) are believed to protect against breast cancer, particularly when exposure occurs during childhood. Little is known about the bioavailability of IFLs in children and how this is affected by oral antibiotics (OABX). We measured IFLs by LC/MS and found that the urinary IFL excretion rate (UIER) reflects circulating IFLs accurately when area-under-curve (AUC) and identical time intervals are used (r=0.93; p<0.001). UIER in children and adults was determined when healthy and when on OABX by collecting urine in pairs of baseline and overnight specimen before and after consuming soy nuts, respectively. Compared to when healthy, children on OABX showed significantly decreased UIER but adults on OABX showed increased UIER (p<0.05). All 37 healthy children showed significantly higher UIERs compared to all 34 healthy adults. UIER is an adequate surrogate for determining IFL bioavailability and for measuring soy or IFL exposure in epidemiologic and other studies.
大豆及其异黄酮（IFLs）被认为可以预防乳腺癌，尤其是在儿童期接触时。关于儿童体内IFLs的生物利用度以及口服抗生素（OABX）如何影响其生物利用度，我们所知甚少。我们通过液相色谱/质谱法测量了IFLs，发现当使用曲线下面积（AUC）和相同时间间隔时，尿中IFL排泄率（UIER）能准确反映循环中的IFLs（r = 0.93；p < 0.001）。分别在食用大豆坚果前后，通过收集基线和过夜样本对尿液，来确定健康儿童和成人以及服用OABX时的UIER。与健康时相比，服用OABX的儿童UIER显著降低，但服用OABX的成人UIER升高（p < 0.05）。所有37名健康儿童的UIER均显著高于所有34名健康成人。UIER是确定IFL生物利用度以及在流行病学和其他研究中测量大豆或IFL暴露的合适替代指标。
