Fernández-Moreno Miguel Angel, Farr Carol L, Kaguni Laurie S, Garesse Rafael
Departamento de Bioquímica, Instituto de Investagaciones Biomédicas Alberto Sols CSIC-UAM, Facultad de Medicina, Universidad Autónoma de Madrid, Spain.
Methods Mol Biol. 2007;372:33-49. doi: 10.1007/978-1-59745-365-3_3.
Mitochondria play an essential role in cellular homeostasis. Although in the last few decades our knowledge of mitochondria has increased substantially, the mechanisms involved in the control of mitochondrial biogenesis remain largely unknown. The powerful genetics of Drosophila combined with a wealth of available cell and molecular biology techniques, make this organism an excellent system to study mitochondria. In this chapter we will review briefly the opportunities that Drosophila offers as a model system and describe in detail how to purify mitochondria from Drosophila and to perform the analysis of developmental gene expression using in situ hybridization.
线粒体在细胞内稳态中起着至关重要的作用。尽管在过去几十年里，我们对线粒体的了解有了显著增加，但线粒体生物发生的调控机制在很大程度上仍不为人知。果蝇强大的遗传学特性，再加上丰富的细胞和分子生物学技术，使这种生物成为研究线粒体的绝佳系统。在本章中，我们将简要回顾果蝇作为模型系统所提供的机会，并详细描述如何从果蝇中纯化线粒体，以及如何使用原位杂交进行发育基因表达分析。
