Karashima Satoshi, Takeuchi Akari, Matsuya Shigeki, Udoh Koh-Ichi, Koyano Kiyoshi, Ishikawa Kunio
Section of Biomaterials, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, 812-8582 Fukuoka, Japan.
J Biomed Mater Res A. 2009 Mar 1;88(3):628-33. doi: 10.1002/jbm.a.31904.
Low-crystallinity hydroxyapatite (HAP) foam is an ideal material for bone substitutes and scaffolds for bone tissue regeneration, because its interconnected pores provide the space for cell growth and tissue penetration, and its composition induces excellent tissue response and good osteoconductivity. In this study, the feasibility of low-crystallinity HAP foam fabrication was evaluated based on the phase transformation reaction or the so-called dissolution-reprecipitation reaction of alpha-tricalcium phosphate (alpha-TCP) foam granules. When alpha-TCP foam granules were placed in water at 37 degrees C for 1 day, no reaction was observed. However, alpha-TCP foam granules set to form low-crystallinity HAP by treating it hydrothermally at 200 degrees C. The network of fully interconnected pores was retained, and porosity was as high as 82%. Pore size ranged from 50 to 300 mum with an average pore size of 160 mum. Compressive strength was 207 kPa. Although no setting reaction was observed at 37 degrees C, the setting reaction caused by the hydrothermal treatment of alpha-TCP foam granules allowed the fabrication of any shape of low-crystallinity HAP. Therefore, this method may be useful for the fabrication of bone substitutes and scaffolds in bone tissue regeneration.
低结晶度羟基磷灰石（HAP）泡沫是骨替代物和骨组织再生支架的理想材料，因为其相互连通的孔隙为细胞生长和组织侵入提供了空间，并且其成分能引发良好的组织反应和骨传导性。在本研究中，基于α-磷酸三钙（α-TCP）泡沫颗粒的相变反应或所谓的溶解-再沉淀反应，评估了制备低结晶度HAP泡沫的可行性。当将α-TCP泡沫颗粒置于37℃的水中1天时，未观察到反应。然而，通过在200℃下进行水热处理，α-TCP泡沫颗粒转变形成了低结晶度HAP。完全相互连通的孔隙网络得以保留，孔隙率高达82%。孔径范围为50至300μm，平均孔径为160μm。抗压强度为207kPa。尽管在37℃下未观察到凝固反应，但对α-TCP泡沫颗粒进行水热处理引发的凝固反应使得能够制备任何形状的低结晶度HAP。因此，该方法可能对骨组织再生中骨替代物和支架的制备有用。
