Sahu Amit Kumar, Quadri Syed Raziuddin, Agasar Dayanand, Ruwaili Jamal Al, Jun-Li Wen, Dastager Syed G
Academy of Scientific and Innovative Research (AcSIR), and NCIM Resource Center, CSIR-National Chemical Laboratory, NCIM Resource Center, Pune, India.
Faculty of Applied Medical Sciences, Department of Medical Laboratory Technology, Northern Border University, Northern Borders, Kingdom of Saudi Arabia.
J Antibiot (Tokyo). 2017 Oct;70(10):1000-1003. doi: 10.1038/ja.2017.82. Epub 2017 Jul 19.
A novel actinobacterium, designated strain YIM 75704, was isolated from a limestone quarry located at Gulbarga, Karnataka, India. The novel strain has showed typical morphological and chemotaxonomic characteristics of the family Streptomycetaceae. Comparison of 16S rRNA gene sequences indicated that this strain represents a novel member of the family Streptomycetaceae and exhibited 99.0% 16S rRNA gene sequence similarities with the type species of the recently described novel genus Allostreptomyces, that is, Allostreptomyces psammosilenae, whereas other species of Streptomyces were below 95% sequence similarity. The cell hydrolysates contained the LL-isomer of diaminopimelic acid and the predominant quinones were MK-9 (H, H and H). The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylinositolmannosides and three unknown phospholipids. The DNA G+C content was 75.0 mol%. A polyphasic study of the strain with morphological, phenotypic, phylogenetic and with DNA-DNA hybridization evidence with related members showed that this strain represents novel species of Allostreptomyces for which the name Allostreptomyces indica sp. nov., is proposed. The type strain is YIM 75704 (= DSM 41985=CCTCC AA 209051= NCIM 5485).
从印度卡纳塔克邦古尔伯加的一个石灰石采石场分离出一株新型放线菌，命名为菌株YIM 75704。该新型菌株表现出链霉菌科典型的形态和化学分类学特征。16S rRNA基因序列比较表明，该菌株代表链霉菌科的一个新成员，与最近描述的新属异链霉菌的模式种，即沙栖异链霉菌的16S rRNA基因序列相似性为99.0%，而链霉菌属的其他物种序列相似性低于95%。细胞水解产物含有二氨基庚二酸的LL-异构体，主要醌类为MK-9（H、H和H）。极性脂质谱由双磷脂酰甘油、磷脂酰肌醇甘露糖苷和三种未知磷脂组成。DNA G+C含量为75.0 mol%。对该菌株进行的多相研究，包括形态学、表型、系统发育以及与相关成员的DNA-DNA杂交证据，表明该菌株代表异链霉菌属的一个新物种，为此提出新名称印度异链霉菌。模式菌株为YIM 75704（=DSM 41985=CCTCC AA 209051=NCIM 5485）。
