Drezek R, Dunn A, Richards-Kortum R
Biomedical Engineering Program, University of Texas at Austin, Engineering Science Building, Room 610, Austin, Texas 78712, USA.
Appl Opt. 1999 Jun 1;38(16):3651-61. doi: 10.1364/ao.38.003651.
We have examined the light-scattering properties of inhomogeneous biological cells through a combination of theoretical simulations and goniometric measurements. A finite-difference time-domain (FDTD) technique was used to compute intensity as a function of scattering angle for cells containing multiple organelles and spatially varying index of refraction profiles. An automated goniometer was constructed to measure the scattering properties of dilute cell suspensions. Measurements compared favorably with FDTD predictions. FDTD and experimental results indicate that scattering properties are strongly influenced by cellular biochemical and morphological structure.
我们通过理论模拟和测角测量相结合的方法，研究了非均匀生物细胞的光散射特性。采用时域有限差分（FDTD）技术，计算含有多个细胞器且折射率分布随空间变化的细胞的散射光强度随散射角的函数关系。构建了一台自动测角仪，用于测量稀细胞悬液的散射特性。测量结果与FDTD预测结果吻合良好。FDTD和实验结果表明，散射特性受细胞生化和形态结构的强烈影响。
