Lewandrowski Urs, Sickmann Albert, Cesaro Luca, Brunati Anna Maria, Toninello Antonio, Salvi Mauro
Protein Mass Spectrometry and Functional Proteomics Group, Rudolf-Virchow-Center for Experimental Biomedicine, Versbacher Strasse 9, 97078 Würzburg, Germany.
FEBS Lett. 2008 Apr 2;582(7):1104-10. doi: 10.1016/j.febslet.2008.02.077. Epub 2008 Mar 10.
Reversible protein-phosphorylation is emerging as a key player in the regulation of mitochondrial functions. In particular tyrosine phosphorylation represents a promising field to highlight new mechanisms of bioenergetic regulation. Utilizing immunoaffinity enrichment of phosphotyrosine-containing peptides coupled to mass spectrometric analysis we detected new tyrosine phosphorylated proteins in rat brain mitochondria after peroxovanadate treatment. By bioinformatic predictions we provide suggestions about the potential role of tyrosine phosphorylation in mitochondrial physiology. Our results indicate a primary role of tyrosine phosphorylation in regulating energy production at the mitochondrial level. Moreover, tyrosine phosphorylation might regulate the mitochondrial membrane permeability targeting protein complexes containing ADP/ATP translocase, VDAC, creatine kinase and hexokinase.
可逆性蛋白质磷酸化正逐渐成为线粒体功能调节中的关键因素。特别是酪氨酸磷酸化，它是揭示生物能量调节新机制的一个很有前景的领域。利用与质谱分析相结合的含磷酸酪氨酸肽段的免疫亲和富集技术，我们在过氧钒酸盐处理后的大鼠脑线粒体中检测到了新的酪氨酸磷酸化蛋白。通过生物信息学预测，我们对酪氨酸磷酸化在线粒体生理学中的潜在作用提出了建议。我们的结果表明酪氨酸磷酸化在调节线粒体水平的能量产生中起主要作用。此外，酪氨酸磷酸化可能通过靶向含有ADP/ATP转位酶、电压依赖性阴离子通道、肌酸激酶和己糖激酶的蛋白质复合物来调节线粒体膜通透性。
