Longo F W, Tomashefsky P, Willhite M, Tannenbaum M
Urology. 1976 Aug;8(2):177-9. doi: 10.1016/0090-4295(76)90353-8.
Paired Wistar-Furth male rats were implanted subcutaneously with the Furth-Columbia rat Wilms' tumor. Seven days after implantation the tumors were either subjected to therapeutic ultrasound irradiation or were left as untreated controls. On the ninth day after implantation the animals were sacrificed, and suspensions of cells were made from the excised tumors by trypsinization. The cells were counted using trypan blue dye as an indicator of viability. New rats were then implanted with cells either from sonicated tumors or cells from controls. In half the recipient animals the suspensions were matched by total cell count. In the other half of the rats cell counts were matches for viability by the dye exclusion test. Tumors developed in a total of 8 of 11 rats receiving control cells and they died. A palpable tumor did not develop in any rat receiving cells fromsonicated tumors during the ninety-day study period. Surviving rats were challenged with Wilms' tumor by trocar implant. Tumor developed in all recipients, and they died.
将成对的雄性Wistar-Furth大鼠皮下植入Furth-Columbia大鼠肾母细胞瘤。植入后7天，对肿瘤进行治疗性超声照射，或留作未治疗的对照。植入后第9天，处死动物，通过胰蛋白酶消化从切除的肿瘤中制备细胞悬液。使用台盼蓝染料作为活力指标对细胞进行计数。然后将新的大鼠植入来自超声处理肿瘤的细胞或对照细胞。在一半的受体动物中，通过总细胞计数使悬液匹配。在另一半大鼠中，通过染料排除试验使细胞计数在活力方面匹配。接受对照细胞的11只大鼠中有8只总共长出了肿瘤，然后死亡。在为期90天的研究期间，接受来自超声处理肿瘤细胞的任何大鼠都没有长出可触及的肿瘤。对存活的大鼠通过套管针植入肾母细胞瘤进行攻击。所有受体都长出了肿瘤，然后死亡。
