Ouwehand Arthur C, Bergsma Nynke, Parhiala Riikka, Lahtinen Sampo, Gueimonde Miguel, Finne-Soveri Harriet, Strandberg Timo, Pitkälä Kaisu, Salminen Seppo
Functional Foods Forum, University of Turku, Turku, Finland.
FEMS Immunol Med Microbiol. 2008 Jun;53(1):18-25. doi: 10.1111/j.1574-695X.2008.00392.x. Epub 2008 Mar 10.
Faecal and serum samples were collected over a period of 6 months from 55 institutionalized elderly subjects, who were enrolled in a double-blind placebo-controlled study. Participants were randomized in one of the three treatment groups: intervention (two probiotic Bifidobacterium longum strains: 2C and 46), placebo and commercial control (Bifidobacterium lactis Bb-12). The faecal Bifidobacterium microbiota was characterized by genus and species-specific PCR. Serum levels of the cytokines IL-10, tumor necrosis factor (TNF)-alpha and transforming growth factor (TGF)-beta1 were determined by enzyme-linked immunosorbent assay. Each participant harboured on average approximately three different bifidobacterial species. The most frequently detected species were B. longum, Bifidobacterium adolescentis and Bifidobacterium bifidum. Depending on the treatment, the intervention resulted in specific changes in the levels of certain Bifidobacterium species, and positive correlations were found between the different species. Negative correlations were observed between the levels of Bifidobacterium species and the pro-inflammatory cytokine TNF-alpha and the regulatory cytokine IL-10. The presence of faecal B. longum and Bifidobacterium animalis correlated with reduced serum IL-10. The anti-inflammatory TGF-beta1 levels were increased over time in all three groups, and the presence of Bifidobacterium breve correlated with higher serum TGF-beta1 levels. This indicates that modulation of the faecal Bifidobacterium microbiota may provide a means of influencing inflammatory responses.
在6个月的时间里，从55名入住养老院的老年受试者中收集粪便和血清样本，这些受试者参与了一项双盲安慰剂对照研究。参与者被随机分为三个治疗组之一：干预组（两种长双歧杆菌菌株：2C和46）、安慰剂组和商业对照组（乳酸双歧杆菌Bb-12）。通过属和种特异性PCR对粪便双歧杆菌微生物群进行表征。通过酶联免疫吸附测定法测定细胞因子IL-10、肿瘤坏死因子（TNF）-α和转化生长因子（TGF）-β1的血清水平。每位参与者平均携带约三种不同的双歧杆菌物种。最常检测到的物种是长双歧杆菌、青春双歧杆菌和两歧双歧杆菌。根据治疗情况，干预导致某些双歧杆菌物种的水平发生特定变化，并且在不同物种之间发现了正相关。在双歧杆菌物种的水平与促炎细胞因子TNF-α和调节性细胞因子IL-10之间观察到负相关。粪便中长双歧杆菌和动物双歧杆菌的存在与血清IL-10降低相关。在所有三个组中，抗炎性TGF-β1水平随时间增加，短双歧杆菌的存在与较高的血清TGF-β1水平相关。这表明调节粪便双歧杆菌微生物群可能提供一种影响炎症反应的手段。
