Ceballos Claire, Prata Carla A H, Giorgio Suzanne, Garzino Frédéric, Payet Dominique, Barthélémy Philippe, Grinstaff Mark W, Camplo Michel
Centre Interdisciplinaire de Nanosciences de Marseille CINaM, UPR-CNRS 3118, Université Aix-Marseille II, Luminy, Case 913, 13288 Marseille Cedex 09, France.
Bioconjug Chem. 2009 Feb;20(2):193-6. doi: 10.1021/bc800432n.
Cationic nucleoside lipids based on a 3-nitropyrrole universal base were prepared from D-ribose using a straightforward chemical synthesis. Several studies including DLS, TEM, and ethidium bromide (EthBr) assay demonstrated that these amphiphilic molecules form supramolecular organizations of nanometer size in aqueous solutions and are able to bind nucleic acids. siRNA knockdown experiments were performed with these nucleolipids, and we observed protein knockdown activity similar to the siPORT NeoFX positive control. No significant cytotoxicity was found.
基于3-硝基吡咯通用碱基的阳离子核苷脂质由D-核糖通过直接化学合成制备而成。包括动态光散射（DLS）、透射电子显微镜（TEM）和溴化乙锭（EthBr）测定在内的多项研究表明，这些两亲性分子在水溶液中形成纳米尺寸的超分子聚集体，并且能够结合核酸。用这些核脂质进行了小干扰RNA（siRNA）敲低实验，我们观察到其蛋白质敲低活性与siPORT NeoFX阳性对照相似。未发现明显的细胞毒性。
