牛冠状病毒非结构蛋白ns2是一种磷蛋白。
Bovine coronavirus nonstructural protein ns2 is a phosphoprotein.
作者信息
Cox G J, Parker M D, Babiuk L A
机构信息
Department of Veterinary Microbiology, University of Saskatchewan, Saskatoon, Canada.
出版信息
Virology. 1991 Nov;185(1):509-12. doi: 10.1016/0042-6822(91)90810-x.
Abstract
To investigate the nature of the bovine coronavirus (BCV) ns2 protein, the gene encoding this protein was cloned and was expressed as a beta-galactosidase fusion protein. Antiserum raised against this protein reacted specifically with BCV-infected fixed cells in indirect immunofluorescence microscopy and precipitated an in vitro synthesized product approximately 32-kDa in molecular weight and an equivalent protein from BCV-infected cells. The synthesis of ns2 was found to be similar to the structural proteins of BCV and pulse-chase experiments indicated that ns2 protein was stable and that it accumulated in BCV-infected cells. Synthesis of ns2 in the presence of [32P] orthophosphate revealed that it is a phosphoprotein. Phosphoamino acid analysis confirmed the phosphorylated nature of ns2 and identified serine and threonine as its phosphorylated amino acid residues. This is the first demonstration of a phosphorylated nonstructural protein in coronavirus-infected cells.
摘要
为了研究牛冠状病毒（BCV）ns2蛋白的性质，编码该蛋白的基因被克隆并表达为β-半乳糖苷酶融合蛋白。用该蛋白制备的抗血清在间接免疫荧光显微镜下与BCV感染的固定细胞发生特异性反应，并沉淀出一种体外合成的分子量约为32 kDa的产物以及来自BCV感染细胞的同等蛋白。发现ns2的合成与BCV的结构蛋白相似，脉冲追踪实验表明ns2蛋白稳定且在BCV感染的细胞中积累。在[32P]正磷酸盐存在的情况下合成ns2表明它是一种磷蛋白。磷酸氨基酸分析证实了ns2的磷酸化性质，并确定丝氨酸和苏氨酸是其磷酸化氨基酸残基。这是首次在冠状病毒感染的细胞中证明存在磷酸化的非结构蛋白。
相似文献
1
Bovine coronavirus nonstructural protein ns2 is a phosphoprotein.牛冠状病毒非结构蛋白ns2是一种磷蛋白。
Virology. 1991 Nov;185(1):509-12. doi: 10.1016/0042-6822(91)90810-x.
2
Sequence and expression analysis of potential nonstructural proteins of 4.9, 4.8, 12.7, and 9.5 kDa encoded between the spike and membrane protein genes of the bovine coronavirus.牛冠状病毒刺突蛋白基因与膜蛋白基因之间编码的4.9 kDa、4.8 kDa、12.7 kDa和9.5 kDa潜在非结构蛋白的序列及表达分析
Virology. 1990 Aug;177(2):488-95. doi: 10.1016/0042-6822(90)90513-q.
3
NS2 protein of influenza virus is found in purified virus and phosphorylated in infected cells.流感病毒的NS2蛋白存在于纯化病毒中，并在受感染细胞中发生磷酸化。
Arch Virol. 1991;116(1-4):69-80. doi: 10.1007/BF01319232.
4
Synthesis of bluetongue virus-encoded phosphoprotein and formation of inclusion bodies by recombinant baculovirus in insect cells: it binds the single-stranded RNA species.蓝舌病毒编码的磷蛋白在昆虫细胞中由重组杆状病毒合成及包涵体的形成：它与单链RNA种类结合。
J Gen Virol. 1990 Sep;71 ( Pt 9):2073-83. doi: 10.1099/0022-1317-71-9-2073.
5
Rotavirus SA11 genome segment 11 protein is a nonstructural phosphoprotein.轮状病毒SA11基因组第11节段蛋白是一种非结构磷蛋白。
J Virol. 1989 Sep;63(9):3974-82. doi: 10.1128/JVI.63.9.3974-3982.1989.
6
Semliki Forest virus-specific non-structural protein nsP3 is a phosphoprotein.Semliki森林病毒特异性非结构蛋白nsP3是一种磷蛋白。
J Gen Virol. 1988 Sep;69 ( Pt 9):2165-78. doi: 10.1099/0022-1317-69-9-2165.
7
Sequence analysis of the turkey enteric coronavirus nucleocapsid and membrane protein genes: a close genomic relationship with bovine coronavirus.火鸡肠道冠状病毒核衣壳蛋白和膜蛋白基因的序列分析：与牛冠状病毒的紧密基因组关系
J Gen Virol. 1991 Jul;72 ( Pt 7):1659-66. doi: 10.1099/0022-1317-72-7-1659.
8
Characterization of the nonstructural and spike proteins of the human respiratory coronavirus OC43: comparison with bovine enteric coronavirus.人呼吸道冠状病毒OC43非结构蛋白和刺突蛋白的特性：与牛肠道冠状病毒的比较
Adv Exp Med Biol. 1993;342:61-7. doi: 10.1007/978-1-4615-2996-5_10.
9
Identification, expression in E. coli and insect cells of the non-structural protein NS2 encoded by mRNA2 of bovine coronavirus (BCV).牛冠状病毒（BCV）mRNA2编码的非结构蛋白NS2的鉴定、在大肠杆菌和昆虫细胞中的表达
Adv Exp Med Biol. 1993;342:69-74. doi: 10.1007/978-1-4615-2996-5_11.
10
Phosphorylation of Sindbis virus nsP3 in vivo and in vitro.辛德毕斯病毒nsP3在体内和体外的磷酸化作用。
Virology. 1990 Nov;179(1):416-27. doi: 10.1016/0042-6822(90)90310-n.
引用本文的文献
1
The Potential Roles of Host Cell miRNAs in Fine-Tuning Bovine Coronavirus (BCoV) Molecular Pathogenesis, Tissue Tropism, and Immune Regulation.宿主细胞微小RNA在微调牛冠状病毒（BCoV）分子发病机制、组织嗜性和免疫调节中的潜在作用
Microorganisms. 2024 Apr 30;12(5):897. doi: 10.3390/microorganisms12050897.
2
Newly identified lineages of porcine hemagglutinating encephalomyelitis virus exhibit respiratory phenotype.新鉴定出的猪血凝性脑脊髓炎病毒谱系表现出呼吸道表型。
Virus Evol. 2023 Aug 18;9(2):vead051. doi: 10.1093/ve/vead051. eCollection 2023.
3
Bovine Coronavirus and the Associated Diseases.牛冠状病毒及相关疾病
Front Vet Sci. 2021 Mar 31;8:643220. doi: 10.3389/fvets.2021.643220. eCollection 2021.
4
The Genome Organization of the Nidovirales: Similarities and Differences between Arteri-, Toro-, and Coronaviruses.尼多病毒目病毒的基因组组织：动脉炎病毒、圆环病毒和冠状病毒之间的异同
Semin Virol. 1997 Feb;8(1):33-47. doi: 10.1006/smvy.1997.0104. Epub 2002 May 25.
5
Organ-specific attenuation of murine hepatitis virus strain A59 by replacement of catalytic residues in the putative viral cyclic phosphodiesterase ns2.通过替换假定的病毒环磷酸二酯酶ns2中的催化残基对鼠肝炎病毒A59株进行器官特异性减毒。
J Virol. 2009 Apr;83(8):3743-53. doi: 10.1128/JVI.02203-08. Epub 2009 Jan 28.
6
Isolation and sequence analysis of canine respiratory coronavirus.犬呼吸道冠状病毒的分离与序列分析
Virus Res. 2007 Mar;124(1-2):78-87. doi: 10.1016/j.virusres.2006.10.004. Epub 2006 Nov 7.
7
Evolutionary history of the closely related group 2 coronaviruses: porcine hemagglutinating encephalomyelitis virus, bovine coronavirus, and human coronavirus OC43.密切相关的2型冠状病毒的进化史：猪血凝性脑脊髓炎病毒、牛冠状病毒和人冠状病毒OC43。
J Virol. 2006 Jul;80(14):7270-4. doi: 10.1128/JVI.02675-05.
8
The group-specific murine coronavirus genes are not essential, but their deletion, by reverse genetics, is attenuating in the natural host.鼠类冠状病毒的群特异性基因并非必需，但通过反向遗传学将其缺失后，在天然宿主中会出现减毒现象。
Virology. 2002 Apr 25;296(1):177-89. doi: 10.1006/viro.2002.1412.
9
Bovine coronaviruses associated with enteric and respiratory diseases in Canadian dairy cattle display different reactivities to anti-HE monoclonal antibodies and distinct amino acid changes in their HE, S and ns4.9 protein.与加拿大奶牛的肠道和呼吸道疾病相关的牛冠状病毒，对抗HE单克隆抗体表现出不同的反应性，并且其HE、S和ns4.9蛋白存在明显的氨基酸变化。
Virus Res. 2001 Jul;76(1):43-57. doi: 10.1016/s0168-1702(01)00243-x.
10
Nucleotide and predicted amino acid sequences of all genes encoded by the 3' genomic portion (9.5 kb) of respiratory bovine coronaviruses and comparisons among respiratory and enteric coronaviruses.牛呼吸道冠状病毒3'基因组部分（9.5 kb）编码的所有基因的核苷酸序列和预测的氨基酸序列，以及呼吸道和肠道冠状病毒之间的比较。
Virus Genes. 1998;17(1):33-42. doi: 10.1023/a:1008048916808.
本文引用的文献
1
Coronavirus JHM: a virion-associated protein kinase.冠状病毒JHM：一种病毒粒子相关蛋白激酶。
J Gen Virol. 1981 Feb;52(Pt 2):235-43. doi: 10.1099/0022-1317-52-2-235.
2
Further characterization of mouse hepatitis virus RNA-dependent RNA polymerases.小鼠肝炎病毒RNA依赖的RNA聚合酶的进一步特性分析。
Virology. 1984 Feb;133(1):197-201. doi: 10.1016/0042-6822(84)90439-2.
3
RNA-dependent RNA polymerase activity in murine coronavirus-infected cells.鼠冠状病毒感染细胞中的RNA依赖性RNA聚合酶活性。
J Gen Virol. 1983 Jan;64 (Pt 1):103-11. doi: 10.1099/0022-1317-64-1-103.
4
DNA is linked to the rat liver DNA nicking-closing enzyme by a phosphodiester bond to tyrosine.DNA通过磷酸二酯键与大鼠肝脏DNA切口封闭酶的酪氨酸相连。
J Biol Chem. 1981 May 25;256(10):4805-9.
5
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.从含有噬菌体SP6启动子的质粒中高效体外合成生物活性RNA和RNA杂交探针。
Nucleic Acids Res. 1984 Sep 25;12(18):7035-56. doi: 10.1093/nar/12.18.7035.
6
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
7
Structural proteins of bovine coronavirus and their intracellular processing.牛冠状病毒的结构蛋白及其细胞内加工过程。
J Gen Virol. 1987 Nov;68 ( Pt 11):2863-77. doi: 10.1099/0022-1317-68-11-2863.
8
Coronaviruses: structure and genome expression.冠状病毒：结构与基因组表达
J Gen Virol. 1988 Dec;69 ( Pt 12):2939-52. doi: 10.1099/0022-1317-69-12-2939.
9
Sequence of mouse hepatitis virus A59 mRNA 2: indications for RNA recombination between coronaviruses and influenza C virus.小鼠肝炎病毒A59 mRNA 2的序列：冠状病毒与丙型流感病毒之间RNA重组的迹象
Virology. 1988 Oct;166(2):415-22. doi: 10.1016/0042-6822(88)90512-0.
10
The sequence of cDNA of bovine coronavirus 32K nonstructural gene.牛冠状病毒32K非结构基因的cDNA序列。
Nucleic Acids Res. 1989 Jul 25;17(14):5847. doi: 10.1093/nar/17.14.5847.
Zotero 插件