Rodrigo Ramón, Prat Hernán, Passalacqua Walter, Araya Julia, Guichard Cristián, Bächler Jean P
Laboratory of Renal Pathophysiology, Molecular and Clinical Pharmacology Program, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Santiago, Chile.
Hypertens Res. 2007 Dec;30(12):1159-67. doi: 10.1291/hypres.30.1159.
This study investigated the association of blood pressure with blood oxidative stress-related parameters in normotensive and hypertensive subjects. A cross-sectional design was applied to 31 hypertensive patients and 35 healthy normotensive subjects. All subjects were men between the ages of 35 and 60 years. Exclusion criteria were obesity, dyslipidemia, diabetes mellitus, smoking and current use of any medication. All patients underwent 24-h ambulatory blood pressure monitoring and sampling of blood and urine. Antioxidant enzymes activity, reduced/oxidized glutathione ratio (GSH/GSSG), and lipid peroxidation (malondialdehyde) were determined in erythrocytes. Parameters measured in the plasma of test subjects were plasma antioxidant status, lipid peroxidation (8-isoprostane), plasma vitamin C and E, and the blood pressure modulators renin, aldosterone, endothelin-1 and homocysteine. Daytime systolic and diastolic blood pressures of hypertensives were negatively correlated with plasma antioxidant capacity (r=-0.46, p<0.009 and r=-0.48, p<0.007), plasma vitamin C levels (r=-0.53, p<0.003 and r=-0.44, p<0.02), erythrocyte activity of antioxidant enzymes, and erythrocyte GSH/GSSG ratio, with hypertensives showing higher levels of oxidative stress. Blood pressures showed a positive correlation with both plasma and urine 8-isoprostane. Neither plasma vitamin E nor the assessed blood pressure modulator levels showed significant differences between the groups or correlation with blood pressures. These findings demonstrate a strong association between blood pressure and some oxidative stress-related parameters and suggest a possible role of oxidative stress in the pathophysiology of essential hypertension.
本研究调查了血压与正常血压和高血压受试者血液氧化应激相关参数之间的关联。采用横断面设计，纳入31例高血压患者和35例健康的正常血压受试者。所有受试者均为35至60岁的男性。排除标准为肥胖、血脂异常、糖尿病、吸烟及目前正在使用任何药物。所有患者均接受24小时动态血压监测以及血液和尿液采样。测定红细胞中的抗氧化酶活性、还原型/氧化型谷胱甘肽比值（GSH/GSSG）和脂质过氧化（丙二醛）。在受试对象的血浆中测定的参数包括血浆抗氧化状态、脂质过氧化（8-异前列腺素）、血浆维生素C和E，以及血压调节因子肾素、醛固酮、内皮素-1和同型半胱氨酸。高血压患者的日间收缩压和舒张压与血浆抗氧化能力呈负相关（r=-0.46，p<0.009和r=-0.48，p<0.007）、与血浆维生素C水平呈负相关（r=-0.53，p<0.003和r=-0.44，p<0.02）、与抗氧化酶的红细胞活性以及红细胞GSH/GSSG比值呈负相关，高血压患者表现出较高的氧化应激水平。血压与血浆和尿液中的8-异前列腺素均呈正相关。血浆维生素E和所评估的血压调节因子水平在两组之间均未显示出显著差异，也与血压无相关性。这些发现表明血压与一些氧化应激相关参数之间存在密切关联，并提示氧化应激在原发性高血压病理生理学中可能发挥作用。
