Kim Yong Hun, Huh Sun, Chung Young Bae
Department of Parasitology, College of Medicine, Hallym University, Chuncheon, Republic of Korea.
Korean J Parasitol. 2008 Mar;46(1):29-32. doi: 10.3347/kjp.2008.46.1.29.
The aim of this study is to determine the Toxocara seropositive rate among healthy people with eosinophilia. A total of 97 people residing in Seoul who were healthy and whose blood eosinophilia was over 10%, as shown by regular health check-ups in 2004, were subjected to this study. Their sera were tested by immunoblotting and ELISA with the antigen of larval Toxocara canis excretory-secretory (ES) protein. Sixty-five sera were band-positive (67.0%). The seropositve control sera were positive to band sizes of 66 kDa, 56 kDa, 32 kDa, and 13 kDa. In ELISA, 63 sera (65.0%) were positive to T. canis ES protein. There was no significant correlation between the IgG ELISA titer and the level of eosinophilia (r = 0.156, P = 0.156). As there were insufficient data to determine whether there were cross-reactions with other helminthic infections, or whether atopy occurred, further studies are required to verify the cause of the seropositive reactions against T. canis ES antigen. Toxocariasis seropositivity is suggested to be the major cause of eosinophilia, since the Toxocara seroprevalence among Korean rural adults was shown to be approximately 5%.
本研究的目的是确定嗜酸性粒细胞增多的健康人群中的弓首线虫血清阳性率。共有97名居住在首尔的健康人参与了本研究，他们在2004年的定期健康检查中显示血液嗜酸性粒细胞增多超过10%。用犬弓首线虫幼虫排泄分泌（ES）蛋白抗原通过免疫印迹法和酶联免疫吸附测定（ELISA）对他们的血清进行检测。65份血清呈条带阳性（67.0%）。血清阳性对照血清对66 kDa、56 kDa、32 kDa和13 kDa的条带大小呈阳性。在ELISA中，63份血清（65.0%）对犬弓首线虫ES蛋白呈阳性。IgG ELISA滴度与嗜酸性粒细胞水平之间无显著相关性（r = 0.156，P = 0.156）。由于没有足够的数据来确定是否与其他蠕虫感染存在交叉反应，或者是否发生特应性反应，因此需要进一步研究以证实针对犬弓首线虫ES抗原的血清阳性反应的原因。鉴于韩国农村成年人中弓首线虫血清阳性率约为5%，弓首线虫病血清阳性被认为是嗜酸性粒细胞增多的主要原因。
