Allahdin Sudabeh, Khademvatan Shahram, Rafiei Abdollah, Momen Aliakbar, Rafiei Reza
Cellular and Molecular Research Center & Dept. Parasitology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Department of Medical Parasitology and Mycology & Cellular and Molecular Research Center, Urmia University of Medical Sciences, Urmia, Iran ; Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Iran J Child Neurol. 2015 Fall;9(4):32-40.
Epilepsy is a disorder of the brain characterized by an enduring predisposition to generate seizures. Infectious agents are mentioned in its etiology. With identifying and appropriate treatment of these infectious agents, preventing their secondary outcomes, including seizure is possible. This study was conducted to determine frequency of anti-Toxoplasma antibodies (IgG, IgM) and anti-Toxocara antibody (IgG) in epileptic patients.
MATERIALS & METHODS: Study sample consisted of 141 epileptic patients and 144 healthy people. After obtaining informed consents and completing demographic questionnaire, serum samples were taken from participants. The diagnostic test of Toxoplasma IgG & IgM and Toxocara antibodies was performed under the same conditions using ELISA method in a qualified private laboratory. Samples from patients and control groups with positive ELISA test in terms of anti-Toxocara antibody were also used for confirmatory Western blot test.
According to ELISA results, 28 (19.85%) epileptic patients and 2(1.38%) of healthy people had anti-Toxocara antibodies (P<001), while 39 (30.46%) of the control group people and 14.18% of patients had anti-Toxoplsma antibodies (P=0.001).
Frequency of anti-Toxoplasma gondii is lower in epileptic than healthy individuals and this result is contrary to investigations that have reported higher levels of this antibody in such patient groups. ELISA results for Toxocara showed that the frequency of anti-Toxocara antibody in epileptic patients might empower the probability that this parasite may cause central nervous system damage. Western blotting has high specificity and is a proper confirmative method for diagnosis of toxocariasis.
癫痫是一种脑部疾病，其特征为具有产生癫痫发作的持久倾向。感染因素在其病因中被提及。通过识别并适当治疗这些感染因素，预防包括癫痫发作在内的继发后果是有可能的。本研究旨在确定癫痫患者中抗弓形虫抗体（IgG、IgM）和抗蛔虫抗体（IgG）的频率。
研究样本包括141例癫痫患者和144名健康人。在获得知情同意并完成人口统计学调查问卷后，采集参与者的血清样本。在一家合格的私人实验室中，使用酶联免疫吸附测定（ELISA）方法在相同条件下进行弓形虫IgG和IgM以及蛔虫抗体的诊断检测。ELISA检测抗蛔虫抗体呈阳性的患者和对照组样本也用于免疫印迹法确证试验。
根据ELISA结果，28例（19.85%）癫痫患者和2例（1.38%）健康人有抗蛔虫抗体（P<0.001），而对照组中有39例（30.46%）和14.18%的患者有抗弓形虫抗体（P=0.001）。
癫痫患者中抗弓形虫的频率低于健康个体，这一结果与报道此类患者群体中该抗体水平较高的研究相反。蛔虫的ELISA结果表明，癫痫患者中抗蛔虫抗体的频率可能增加了这种寄生虫可能导致中枢神经系统损害的可能性。免疫印迹法具有高特异性，是诊断蛔虫病的一种合适的确证方法。
