Chen Hsiao-Jan, Tsai Jui-Chang, Chang Tsung-Chain, Hung Wei-Chun, Tseng Sung-Pin, Hsueh Po-Ren, Teng Lee-Jene
Department of Clinical Laboratory Sciences and Medical Biotechnology, National Taiwan University College of Medicine, Taipei, Taiwan.
Division of Neurosurgery, Department of Surgery, National Taiwan University Hospital, Taipei, Taiwan.
J Med Microbiol. 2008 Apr;57(Pt 4):432-438. doi: 10.1099/jmm.0.47628-0.
The sequence diversity of groESL genes among Streptococcus bovis group isolates was analysed, including five reference strains and 36 clinical isolates. Phylogenetic analysis of the groES and groEL sequences showed that the isolates that belonged to the same species or subspecies usually clustered together. The intergenic spacer region between groES and groEL was variable in size (67-342 bp) and sequence and appeared to be a unique marker for species or subspecies determination. Sequence similarities of the groESL genes among species and subspecies ranged from 84.2 to 99.0 % in groES, and from 88.0 to 99.0 % in groEL. Based on the sequences determined, a Streptococcus bovis group-specific PCR assay was developed, which may provide an alternative means of distinguishing the bovis group from other viridans streptococci. Restriction digestion of the amplicon with AclI further differentiated the species and subspecies.
分析了牛链球菌群分离株中groESL基因的序列多样性，包括5株参考菌株和36株临床分离株。groES和groEL序列的系统发育分析表明，属于同一物种或亚种的分离株通常聚集在一起。groES和groEL之间的基因间隔区大小（67 - 342 bp）和序列可变，似乎是用于物种或亚种鉴定的独特标记。物种和亚种之间groESL基因的序列相似性在groES中为84.2%至99.0%，在groEL中为88.0%至99.0%。基于所确定的序列，开发了一种牛链球菌群特异性PCR检测方法，这可能为区分牛链球菌群与其他草绿色链球菌提供一种替代方法。用AclI对扩增子进行限制性消化可进一步区分物种和亚种。
