利用口腔液样本对近期腮腺炎暴发进行的流行病学和诊断评估。
Epidemiologic and diagnostic evaluation of a recent mumps outbreak using oral fluid samples.
作者信息
Reid Fiona, Hassan Jaythoon, Irwin Fiona, Waters Allison, Hall William, Connell Jeff
机构信息
National Virus Reference Laboratory, School of Medicine and Medical Sciences, University College Dublin, Belfield, Dublin 4, Ireland.
出版信息
J Clin Virol. 2008 Feb;41(2):134-7. doi: 10.1016/j.jcv.2007.10.009.
OBJECTIVE
To determine the optimal strategy to investigate mumps virus infection in a partially vaccinated cohort.
STUDY DESIGN
122 oral fluid and serum samples were collected in a recent outbreak in Ireland. The largest age cohort, students aged 18-21 years old attending third level institutions, were investigated using virus isolation, detection of mumps specific IgM, IgG, RT-PCR and molecular genotyping.
RESULTS
97% of patients had both detectable serum IgM and IgG. Mumps virus RNA was detected in 17 oral fluid samples and 14 of these originated from a single geographic location. Only 6 of the IgM positive samples had detectable mumps virus RNA whereas this could be detected in 11 IgM negative samples. Genotyping studies revealed that genotypes G and J were co-circulating during this outbreak.
CONCLUSIONS
The use of an oral fluid sample to detect mumps virus RNA and IgM offers a major improvement over serological diagnosis in acute infection in both non-vaccinated or partially vaccinated individuals, and has the advantage that specimens are collected non-invasively.
目的
确定在部分接种疫苗人群中调查腮腺炎病毒感染的最佳策略。
研究设计
在爱尔兰最近一次疫情中收集了122份口腔液和血清样本。对最大年龄组，即就读于第三级机构的18 - 21岁学生，采用病毒分离、腮腺炎特异性IgM、IgG检测、逆转录聚合酶链反应（RT-PCR）和分子基因分型进行调查。
结果
97%的患者血清中均可检测到IgM和IgG。在17份口腔液样本中检测到腮腺炎病毒RNA，其中14份来自单一地理位置。仅6份IgM阳性样本可检测到腮腺炎病毒RNA，而在11份IgM阴性样本中可检测到该病毒RNA。基因分型研究表明，在此次疫情期间基因型G和J共同传播。
结论
使用口腔液样本检测腮腺炎病毒RNA和IgM，相较于非接种或部分接种个体急性感染时的血清学诊断有重大改进，且具有样本采集无创的优势。
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