von Eiff Christof, Maas Dominik, Sander Gunnar, Friedrich Alexander W, Peters Georg, Becker Karsten
Institute of Medical Microbiology, University Hospital of Münster, Domagkstr. 10, 48149 Münster, Germany.
J Antimicrob Chemother. 2008 Jun;61(6):1277-80. doi: 10.1093/jac/dkn122. Epub 2008 Mar 20.
Recently, a rapid screening tool for methicillin-resistant Staphylococcus aureus (MRSA) has been introduced that applies a novel detection technology allowing the rapid presence or absence of MRSA to be determined from an enrichment broth after only a few hours of incubation. To evaluate the reliability of this new assay to successfully detect MRSA strains of different origin and clonality, well-characterized S. aureus strains were tested in this study.
More than 700 methicillin-susceptible and methicillin-resistant strains covering >90% of all registered European MRSA spa types within the SeqNet network were studied.
All 513 MRSA strains tested were recognized as methicillin-resistant: among these, 96 MRSA strains were from an institutional collection, each presenting a unique spa type. None of the 211 methicillin-susceptible strains were detected as positive.
The new growth-based rapid MRSA assay was shown to detect without exception all MRSA strains of large collections of strains comprising highly diverse genetic backgrounds, indicating that such a phenotypic test might be potentially more likely to cope with new strains.
最近，一种用于耐甲氧西林金黄色葡萄球菌（MRSA）的快速筛查工具被引入，该工具应用了一种新型检测技术，只需经过数小时培养，就能从富集肉汤中快速确定MRSA的有无。为评估这种新检测方法成功检测不同来源和克隆性的MRSA菌株的可靠性，本研究对特征明确的金黄色葡萄球菌菌株进行了检测。
研究了700多株甲氧西林敏感和耐药菌株，这些菌株涵盖了SeqNet网络中所有已注册的欧洲MRSA spa型的90%以上。
所检测的513株MRSA菌株均被识别为耐甲氧西林：其中96株MRSA菌株来自机构收集，每株呈现独特的spa型。211株甲氧西林敏感菌株均未检测为阳性。
新的基于生长的MRSA快速检测方法被证明能无一例外地检测出大量具有高度多样遗传背景的菌株中的所有MRSA菌株，这表明这种表型检测可能更有可能应对新菌株。
