Zellweger M, Goujon D, Conde R, Forrer M, van den Bergh H, Wagnières G
Appl Opt. 2001 Aug 1;40(22):3784-91. doi: 10.1364/ao.40.003784.
Autofluorescence is emerging as a useful tool for the detection of early cancers in the bronchi. It has already produced interesting results, which have been implemented in commercial imaging devices. Their design relies on the spectroscopy of the tissues of interest. However, a large majority of these autofluorescence spectroscopy studies have been presented in arbitrary units. This is a drawback for, in particular, the designing of imaging devices based on autofluorescence. Using correction factors and a spectral sensitivity correction curve, we determined the absolute spectral distribution of the tissue autofluorescence in vivo. These measurements were performed on healthy, metaplastic, and dysplastic bronchial tissues at several excitation wavelengths ranging from 350 to 495 nm. Moreover, we measured at a fixed distance between the tissue and the probe to avoid geometric distortions of the spectra that are due to the optical characteristics of tissue. We found that the order of magnitude of the autofluorescence brightness was stable as the excitation wavelengths varied (on the order of 5 pW/muW x nm at the maximum of the fluorescence emission spectra).
自体荧光正逐渐成为检测支气管早期癌症的一种有用工具。它已经产生了有趣的结果，并已应用于商业成像设备中。这些设备的设计依赖于感兴趣组织的光谱学。然而，这些自体荧光光谱研究中的大部分都是以任意单位呈现的。这对于基于自体荧光的成像设备的设计来说尤其是个缺点。通过使用校正因子和光谱灵敏度校正曲线，我们确定了体内组织自体荧光的绝对光谱分布。这些测量是在健康、化生和发育异常的支气管组织上进行的，激发波长范围为350至495纳米。此外，我们在组织与探头之间的固定距离处进行测量，以避免由于组织的光学特性导致的光谱几何失真。我们发现，随着激发波长的变化，自体荧光亮度的数量级是稳定的（在荧光发射光谱的最大值处约为5皮瓦/微瓦×纳米）。
