Luukkaa Heikki, Klemi Pekka, Hirsimäki Pirkko, Vahlberg Tero, Kivisaari Atte, Kähäri Veli-Matti, Grénman Reidar
Department of Otorhinolaryngology-Head and Neck Surgery, University of Turku, Turku, Finland.
Acta Otolaryngol. 2008 Apr;128(4):482-90. doi: 10.1080/00016480801922895.
In the current study matrix metalloproteinases (MMPs)-9 and -13 were associated with the prognosis in salivary gland cancer (SGC), indicating that they contribute to the progression and invasion of these malignant tumours.
Elevated levels of certain MMPs have been detected in various advanced human cancer types. The purpose of the study was to analyse the expression of MMP-1, -9 and -13 in SGC by immunohistochemistry and correlate the results to the clinical data and 10-year survival of SGC patients in a nationwide material.
Immunohistochemistry for MMP-1, -9 and -13 was performed in series of 103 paraffin-embedded sections of SGC.
High MMP-13 staining intensity predicted poor survival (3 vs 1; p=0.08) in the whole material studied. High MMP-13 intensity (2+3 vs 1; p=0.05), percentage (2 vs 1; p=0.03) and index (3 vs 1; p=0.02) were associated with poor survival in acinic cell carcinoma. However, high MMP-9 index in adenoid cystic carcinoma (p=0.06) and the percentage of positively staining cells in salivary duct carcinoma patients (p=0.05) was associated with poor survival. High MMP-1 staining intensity (2 vs 0; p=0.06) and index (% x intensity); (2 vs 1, p=0.04) were associated with better overall survival in the whole material.
在本研究中，基质金属蛋白酶（MMPs）-9和-13与涎腺癌（SGC）的预后相关，表明它们促进了这些恶性肿瘤的进展和侵袭。
在多种晚期人类癌症类型中已检测到某些MMPs水平升高。本研究的目的是通过免疫组织化学分析MMP-1、-9和-13在SGC中的表达，并将结果与全国范围内的临床数据及SGC患者的10年生存率相关联。
对103例SGC石蜡包埋切片进行MMP-1、-9和-13的免疫组织化学检测。
在所研究的全部样本中，MMP-13高染色强度预示着较差的生存率（3比1；p = 0.08）。在腺泡细胞癌中，MMP-13高染色强度（2 + 3比1；p = 0.05）、高百分比（2比1；p = 0.03）和高指数（3比1；p = 0.0）与较差的生存率相关。然而，在腺样囊性癌中，MMP-9高指数（p = 0.06）以及在涎腺导管癌患者中阳性染色细胞的百分比（p = 0.05）与较差的生存率相关。在全部样本中，MMP-1高染色强度（2比0；p = 0.06）和指数（百分比×强度）；（2比1，p = 0.04）与较好的总生存率相关。
