Redondo-Nieto Miguel, Lloret Javier, Larenas Javiera, Barahona Emma, Navazo Ana, Martínez-Granero Francisco, Capdevila Silvia, Rivilla Rafael, Martín Marta
Departamento de Biología, Universidad Autónoma de Madrid, 28049 Madrid, Spain.
J Bacteriol. 2008 Jun;190(11):4106-9. doi: 10.1128/JB.00178-08. Epub 2008 Mar 28.
Pseudomonas fluorescens F113 is motile by means of type b flagella. Analysis of the region encoding the synthesis of the flagellar filament has shown a transcriptional organization different from that of type a flagella. Additionally to the promoters driving fliC, fliD, and fleQ expression, we have found promoters upstream of the flaG gene and the fliST operon. These promoters were functional in vivo. Both promoters have been mapped and appear to be dependent on the vegetative sigma factor and independent of FleQ, the master regulator of flagellum synthesis.
荧光假单胞菌F113借助b型鞭毛运动。对编码鞭毛丝合成区域的分析表明,其转录组织不同于a型鞭毛。除了驱动fliC、fliD和fleQ表达的启动子外,我们还在flaG基因和fliST操纵子的上游发现了启动子。这些启动子在体内具有功能。两个启动子都已定位,似乎依赖于营养型σ因子,且独立于鞭毛合成的主要调节因子FleQ。