Ferro M E, Romero-Piffiguer M, Rivero V, Yranzo-Volonte N, Correa S, Riera C M
Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba, Argentina.
Autoimmunity. 1991;9(3):193-200. doi: 10.3109/08916939109007644.
In the present study, we report that Cy-sensitive, MRAG-adherent spleen mononuclear (SpM) inductor-phase T suppressor (Ts) cells obtained from rats pretreated with low doses of a purified fraction (FI) of rat male accessory gland antigens (RAG) are mainly OX19+ and W3/25+. Furthermore, thymocytes from rats pretreated with FI of RAG restore the suppression of the autoimmune response to RAG autoantigens in irradiated recipients of SpM inductor-phase Ts cells. In contrast, thymocytes from rats pretreated with rat heart saline extract (unrelated antigen) did not recuperate the suppression of the autoimmune response detected by macrophage migration inhibitory factor (MIF) and delayed-type hypersensitivity. The suppressor thymocytes did not directly exert their inhibitory effect because they were not effective to suppress the autoimmune response to RAG autoantigens when irradiated recipients did not receive SpM inductor-phase Ts cells. The effect of these thymocytes was found in PNA--but not in PNA+ thymic cell population. The perithymic injection of Toxoplasma gondii did block their suppressor activity. The present report clearly shows an active participation of thymus in the efferent phase of the suppressor circuit that controls the autoimmune response to MRAG. The implications of these findings are discussed.
在本研究中，我们报告称，从用低剂量大鼠雄性附属性腺抗原（RAG）的纯化组分（FI）预处理的大鼠中获得的对环磷酰胺敏感、可黏附于MRAG的脾脏单核（SpM）诱导期T抑制（Ts）细胞主要为OX19 +和W3/25 +。此外，用RAG的FI预处理的大鼠的胸腺细胞可恢复对接受SpM诱导期Ts细胞的受辐照受体中针对RAG自身抗原的自身免疫反应的抑制作用。相比之下，用大鼠心脏盐水提取物（无关抗原）预处理的大鼠的胸腺细胞不能恢复通过巨噬细胞移动抑制因子（MIF）和迟发型超敏反应检测到的自身免疫反应的抑制作用。抑制性胸腺细胞不会直接发挥其抑制作用，因为当受辐照受体未接受SpM诱导期Ts细胞时，它们对抑制针对RAG自身抗原的自身免疫反应无效。这些胸腺细胞的作用在PNA -而非PNA +胸腺细胞群体中被发现。胸腺周围注射弓形虫确实会阻断它们的抑制活性。本报告清楚地表明胸腺在控制针对MRAG的自身免疫反应的抑制回路的传出阶段中发挥了积极作用。讨论了这些发现的意义。
