Cha Sangwon, Zhang Hui, Ilarslan Hilal I, Wurtele Eve Syrkin, Brachova Libuse, Nikolau Basil J, Yeung Edward S
Ames Laboratory-USDOE and Department of Chemistry, Iowa State University, Ames, IA 50011, USA.
Plant J. 2008 Jul;55(2):348-60. doi: 10.1111/j.1365-313X.2008.03507.x. Epub 2008 Apr 4.
Laser desorption/ionization (LDI)-based imaging mass spectrometry (MS) has been applied to several biological systems to obtain information about both the identities of the major chemical species and their localization. Colloidal graphite-assisted LDI (GALDI) MS imaging was introduced for the imaging of small molecules such as phospholipids, cerebrosides, oligosaccharides, flavonoids, and other secondary metabolites with high spatial homogeneity due to finely dispersed particles. Mass profiles and images of Arabidopsis thaliana have been recorded directly from various plant surfaces and cross sections. The main targeted metabolites were flavonoids and cuticular waxes, both of which are important in many aspects of functional genomics, proteomics, and metabolomics. The mass spectral profiles revealed tissue-specific accumulation of flavonoids in flowers and petals. In addition, many other location-specific ions were observed. The location and the degree of light-induced accumulation of flavonoids in stem sections were successfully probed by GALDI MS.
基于激光解吸/电离（LDI）的成像质谱（MS）已应用于多个生物系统，以获取有关主要化学物质的身份及其定位的信息。由于颗粒分散精细，胶体石墨辅助LDI（GALDI）MS成像被引入用于对小分子如磷脂、脑苷脂、寡糖、黄酮类化合物和其他次生代谢物进行具有高空间均匀性的成像。拟南芥的质谱图和图像已直接从各种植物表面和横截面记录下来。主要的目标代谢物是黄酮类化合物和表皮蜡质，这两者在功能基因组学、蛋白质组学和代谢组学的许多方面都很重要。质谱图揭示了黄酮类化合物在花和花瓣中的组织特异性积累。此外，还观察到许多其他位置特异性离子。通过GALDI MS成功探测了茎段中黄酮类化合物的光诱导积累位置和程度。
