Khantisitthiporn Onruedee, Sookrung Nitat, Tungtrongchitr Anchalee, Tongtawe Pongsri, Bunnag Chaweewan, Srimanote Potjanee, Tapchaisri Pramuan, Chaicumpa Wanpen
Faculty of Allied Health Sciences, Thammasat University, Rangsit Center, Pathumthani, Thailand.
Asian Pac J Allergy Immunol. 2007 Dec;25(4):189-97.
The American cockroach, Periplaneta americana, is the predominant cockroach (CR) species in Thailand and a major source of indoor allergens second only to the house dust mite. The incidence of CR allergy among allergic Thai patients is increasing but basic information on the allergenic components is scarce. In this study a recombinant troponin-T was produced by using cDNA prepared from RNA of the P. americana as a template and PCR primers designed from the P. americana troponin-T sequence deposited in the GenBank database. The recombinant protein (Mr approximately 50) did not bind to IgE in the sera of 18 skin prick test positive CR allergic patients. Rabbit polyclonal antiserum (PAb) against the recombinant troponin-T was produced and used in preparing an affinity column for the purification of native troponin-T from the crude P. americana extract (Mr approximately 47). IgE-immunoblotting revealed that the native protein bound to IgE in 3 of the 18 (16.7%) patients. Our results imply that native P. americana troponin-T, but not its recombinant counterpart, is a minor allergen among the CR allergic Thais.
美国蟑螂（美洲大蠊）是泰国主要的蟑螂种类，也是仅次于屋尘螨的室内过敏原的主要来源。泰国过敏患者中蟑螂过敏的发生率正在上升，但关于过敏原成分的基础信息却很匮乏。在本研究中，以从美洲大蠊RNA制备的cDNA为模板，使用根据GenBank数据库中美洲大蠊肌钙蛋白-T序列设计的PCR引物，制备了重组肌钙蛋白-T。该重组蛋白（分子量约为50）与18名皮肤点刺试验阳性的蟑螂过敏患者血清中的IgE不结合。制备了针对重组肌钙蛋白-T的兔多克隆抗血清（PAb），并用于制备亲和柱，以从美洲大蠊粗提物（分子量约为47）中纯化天然肌钙蛋白-T。IgE免疫印迹显示，18名患者中有3名（16.7%）的天然蛋白与IgE结合。我们的结果表明，在泰国蟑螂过敏患者中，天然美洲大蠊肌钙蛋白-T是一种次要过敏原，但其重组对应物不是。
